Titre
Combination of Synthetic Long Peptides and XCL1 Fusion Proteins Results in Superior Tumor Control.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Botelho, N.K.
Auteure/Auteur
Tschumi, B.O.
Auteure/Auteur
Hubbell, J.A.
Auteure/Auteur
Swartz, M.A.
Auteure/Auteur
Donda, A.
Auteure/Auteur
Romero, P.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1664-3224
Statut éditorial
Publié
Date de publication
2019
Volume
10
Première page
294
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
Cross-presenting Xcr1 <sup>+</sup> CD8α DCs are attractive APCs to target for therapeutic cancer vaccines, as they are able to take up and process antigen from dying tumor cells for their MHCI-restricted presentation to CD8 T cells. To this aim, we developed fusion proteins made of the Xcr1 ligand Xcl1 fused to an OVA synthetic long peptide (SLP) and IgG1 Fc fragment. We demonstrated the specific binding and uptake of the Xcl1 fusion proteins by Xcr1 <sup>+</sup> DCs. Most importantly, their potent adjuvant effect on the H-2Kb/OVA specific T cell response was associated with a sustained tumor control even against the poorly immunogenic B16-OVA melanoma tumor. The increased tumor protection correlated with higher tumor infiltration of antigen-specific CD8+ T cells, increased IFNγ production and degranulation potential. Altogether, these results demonstrate that therapeutic cancer vaccines may be greatly improved by the combination of SLP antigen and Xcl1 fusion proteins.
PID Serval
serval:BIB_6975012892E2
PMID
Open Access
Oui
Date de création
2019-03-11T16:09:31.216Z
Date de création dans IRIS
2025-05-20T19:00:10Z
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Nom
30863405_BIB_6975012892E2.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by/4.0
Taille
3.03 MB
Format
Adobe PDF
PID Serval
serval:BIB_6975012892E2.P001
URN
urn:nbn:ch:serval-BIB_6975012892E25
Somme de contrôle
(MD5):3af938972cff1492520b0160e1c436c0