Titre
Novel microsatellite loci for Sebaea aurea (Gentianaceae) and cross-amplification in related species.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Kissling, J.
Auteure/Auteur
Bachmann, O.
Auteure/Auteur
Thali, M.R.
Auteure/Auteur
Segarra-Moragues, J.G.
Auteure/Auteur
Liens vers les unités
ISSN
2168-0450
Statut éditorial
Publié
Date de publication
2013
Volume
1
Numéro
12
Première page
1300056
Langue
anglais
Résumé
Premise of the study: Microsatellite loci were developed in Sebaea aurea (Gentianaceae) to investigate the functional role of diplostigmaty (i.e., the presence of additional stigmas along the style).
Methods and Results: One hundred seventy-four and 180 microsatellite loci were isolated through 454 shotgun sequencing of genomic and microsatellite-enriched DNA libraries, respectively. Sixteen polymorphic microsatellite loci were characterized, and 12 of them were selected to genotype individuals from two populations. Microsatellite amplification was conducted in two multiplex groups, each containing six microsatellite loci. Cross-species amplification was tested in seven other species of Sebaea. The 12 novel microsatellite loci amplified only in the two most closely related species to S. aurea (i.e., S. ambigua and S. minutiflora) and were also polymorphic in these two species.
Conclusions: These results demonstrate the usefulness of this set of newly developed microsatellite loci to investigate the mating system and population genetic structure in S. aurea and related species.
Methods and Results: One hundred seventy-four and 180 microsatellite loci were isolated through 454 shotgun sequencing of genomic and microsatellite-enriched DNA libraries, respectively. Sixteen polymorphic microsatellite loci were characterized, and 12 of them were selected to genotype individuals from two populations. Microsatellite amplification was conducted in two multiplex groups, each containing six microsatellite loci. Cross-species amplification was tested in seven other species of Sebaea. The 12 novel microsatellite loci amplified only in the two most closely related species to S. aurea (i.e., S. ambigua and S. minutiflora) and were also polymorphic in these two species.
Conclusions: These results demonstrate the usefulness of this set of newly developed microsatellite loci to investigate the mating system and population genetic structure in S. aurea and related species.
PID Serval
serval:BIB_41CEA22E43C6
PMID
Open Access
Oui
Date de création
2014-10-30T09:34:09.575Z
Date de création dans IRIS
2025-05-20T19:46:41Z
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Nom
BIB_41CEA22E43C6.P001.pdf
Version du manuscrit
preprint
Taille
497.75 KB
Format
Adobe PDF
PID Serval
serval:BIB_41CEA22E43C6.P001
URN
urn:nbn:ch:serval-BIB_41CEA22E43C60
Somme de contrôle
(MD5):fdefdeaadc96e9bcc76f492f03bbb17f