Titre
An integrated pipeline for comprehensive analysis of immune cells in human brain tumor clinical samples.
Type
synthèse (review)
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Maas, R.R.
Auteure/Auteur
Soukup, K.
Auteure/Auteur
Klemm, F.
Auteure/Auteur
Kornete, M.
Auteure/Auteur
Bowman, R.L.
Auteure/Auteur
Bedel, R.
Auteure/Auteur
Marie, D.N.
Auteure/Auteur
Álvarez-Prado, Á.F.
Auteure/Auteur
Labes, D.
Auteure/Auteur
Wilson, A.
Auteure/Auteur
Brouland, J.P.
Auteure/Auteur
Daniel, R.T.
Auteure/Auteur
Hegi, M.E.
Auteure/Auteur
Joyce, J.A.
Auteure/Auteur
Liens vers les personnes
ISSN
1750-2799
Statut éditorial
Publié
Date de publication
2021-10
Volume
16
Numéro
10
Première page
4692
Dernière page/numéro d’article
4721
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Review
Publication Status: ppublish
Publication Status: ppublish
Résumé
Human tissue samples represent an invaluable source of information for the analysis of disease-specific cellular alterations and their variation between different pathologies. In cancer research, advancing a comprehensive understanding of the unique characteristics of individual tumor types and their microenvironment is of considerable importance for clinical translation. However, investigating human brain tumor tissue is challenging due to the often-limited availability of surgical specimens. Here we describe a multimodule integrated pipeline for the processing of freshly resected human brain tumor tissue and matched blood that enables analysis of the tumor microenvironment, with a particular focus on the tumor immune microenvironment (TIME). The protocol maximizes the information yield from limited tissue and includes both the preservation of bulk tissue, which can be performed within 1 h following surgical resection, as well as tissue dissociation for an in-depth characterization of individual TIME cell populations, which typically takes several hours depending on tissue quantity and further downstream processing. We also describe integrated modules for immunofluorescent staining of sectioned tissue, bulk tissue genomic analysis and fluorescence- or magnetic-activated cell sorting of digested tissue for subsequent culture or transcriptomic analysis by RNA sequencing. Applying this pipeline, we have previously described the overall TIME landscape across different human brain malignancies, and were able to delineate disease-specific alterations of tissue-resident versus recruited macrophage populations. This protocol will enable researchers to use this pipeline to address further research questions regarding the tumor microenvironment.
PID Serval
serval:BIB_56ABDAED12F3
PMID
Date de création
2021-09-01T13:31:02.603Z
Date de création dans IRIS
2025-05-20T15:33:18Z
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Nom
s41596-021-00594-2.pdf
Version du manuscrit
published
Licence
https://iris.unil.ch/disclaimer
Taille
6.05 MB
Format
Adobe PDF
PID Serval
serval:BIB_56ABDAED12F3.P001
Somme de contrôle
(MD5):98a1768b937cc18bb43972bb2072e5d7