Titre
An infrared reflection-absorption spectroscopic (IRRAS) study of the interaction of lipid A and lipopolysaccharide Re with endotoxin-binding proteins.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Kerth, A.
Auteure/Auteur
Garidel, P.
Auteure/Auteur
Howe, J.
Auteure/Auteur
Alexander, C.
Auteure/Auteur
Mach, J.P.
Auteure/Auteur
Waelli, T.
Auteure/Auteur
Blume, A.
Auteure/Auteur
Rietschel, E.Th.
Auteure/Auteur
Brandenburg, K.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1875-6638
Statut éditorial
Publié
Date de publication
2009
Volume
5
Numéro
6
Première page
535
Dernière page/numéro d’article
542
Langue
anglais
Résumé
Lipopolysaccharides (LPS, endotoxins) are main constituents of the outer membranes of Gram-negative bacteria, with the 'endotoxic principle' lipid A anchoring LPS into the membrane. When LPS is removed from the bacteria by the action of the immune system or simply by cell dividing, it may interact strongly with immunocompetent cells such as mononuclear cells. This interaction may lead, depending on the LPS concentration, to beneficial (at low) or pathophysiological (at high concentrations) reactions, the latter frequently causing the septic shock syndrome. There is a variety of endogenous LPS-binding proteins. To this class belong lactoferrin (LF) and hemoglobin (Hb), which have been shown to suppress and enhance the LPS-induced cytokine secretion in mononuclear cells, respectively. To elucidate the interaction mechanisms of endotoxins with these proteins, we have investigated in an infrared reflection-absorption spectroscopy (IRRAS) study the interaction of LPS or lipid A monolayers at the air/water interface with LF and Hb proteins, injected into the aqueous subphase. The data are clearly indicative of completely different interaction mechanisms of the endotoxins with the proteins, with the LF acting only at the LPS backbone, whereas Hb incorporates into the lipid monolayer. These data allow an understanding of the different reactivities in the biomedicinal systems.
PID Serval
serval:BIB_20364C9D8C5A
PMID
Date de création
2010-02-02T13:06:01.706Z
Date de création dans IRIS
2025-05-20T16:48:39Z