Titre
Suppression of short interfering RNA-mediated gene silencing by the structural proteins of hepatitis C virus.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Ji, J.
Auteure/Auteur
Glaser, A.
Auteure/Auteur
Wernli, M.
Auteure/Auteur
Berke, J.M.
Auteure/Auteur
Moradpour, D.
Auteure/Auteur
Erb, P.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0022-1317
Statut éditorial
Publié
Date de publication
2008
Volume
89
Numéro
Pt 11
Première page
2761
Dernière page/numéro d’article
2766
Peer-reviewed
Oui
Langue
anglais
Résumé
Viruses have evolved strategies to overcome the antiviral effects of the host at different levels. Besides specific defence mechanisms, the host responds to viral infection via the interferon pathway and also by RNA interference (RNAi). However, several viruses have been identified that suppress RNAi. We addressed the question of whether hepatitis C virus (HCV) suppresses RNAi, using cell lines constitutively expressing green fluorescent protein (GFP) and inducibly expressing HCV proteins. It was found that short interfering RNA-mediated GFP gene silencing was inhibited when the entire HCV polyprotein was expressed. Further studies showed that HCV structural proteins, and in particular envelope protein 2 (E2), were responsible for this inhibition. Co-precipitation assays demonstrated that E2 bound to Argonaute-2 (Ago-2), a member of the RNA-induced silencing complex, RISC. Thus, HCV E2 that interacts with Ago-2 is able to suppress RNAi.
PID Serval
serval:BIB_53483B727194
PMID
Open Access
Oui
Date de création
2009-03-02T12:50:36.662Z
Date de création dans IRIS
2025-05-20T15:01:07Z