Titre
Miniaturized electrochemical biosensor based on whole-cell for heavy metal ions detection in water.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Sciuto, E.L.
Auteure/Auteur
Petralia, S.
Auteure/Auteur
van der Meer, J.R.
Auteure/Auteur
Conoci, S.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1097-0290
Statut éditorial
Publié
Date de publication
2021-04
Volume
118
Numéro
4
Première page
1456
Dernière page/numéro d’article
1465
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
The heavy metals pollution represents one of the important issues in the environmental field since it is involved in many pathologies from cancer, neurodegenerative, and metabolic diseases. We propose an innovative portable biosensor for the determination of traces of trivalent arsenic (As(III)) and bivalent mercury (Hg(II)) in water. The system implements a strategy combining two advanced sensing modules consisting in (a) a whole cell based on engineered Escherichia coli as selective sensing element towards the metals and (b) an electrochemical miniaturised silicon device with three microelectrodes and a portable reading system. The sensing mechanism relies on the selective recognition from the bacterium of given metals producing the 4-aminophenol redox active mediator detected through a cyclic voltammetry analysis. The miniaturized biosensor is able to operate a portable, robust, and high-sensitivity detection of As(III) with a sensitivity of 0.122 µA ppb <sup>-1</sup> , LoD of 1.5 ppb, and a LoQ of 5 ppb. The LoD value is one order of magnitude below of the value indicated to WHO to be dangerous (10 μg/L). The system was proved to be fully versatile being effective in the detection of Hg(II) as well. A first study on Hg(II) showed sensitivity value of 2.11 µA/ppb a LOD value of 0.1 ppb and LoQ value of 0.34 ppb. Also in this case, the detected LOD was 10 times lower than that indicated by WHO (1 ppb). These results pave the way for advanced sensing strategies suitable for the environmental monitoring and the public safety.
PID Serval
serval:BIB_99F692FA2472
PMID
Date de création
2020-12-21T13:31:56.290Z
Date de création dans IRIS
2025-05-21T02:21:56Z