Titre
A joint-ParB interface promotes Smc DNA recruitment.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Bock, F.P.
Auteure/Auteur
Liu, H.W.
Auteure/Auteur
Anchimiuk, A.
Auteure/Auteur
Diebold-Durand, M.L.
Auteure/Auteur
Gruber, S.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
2211-1247
Statut éditorial
Publié
Date de publication
2022-08-30
Volume
40
Numéro
9
Première page
111273
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Chromosomes readily unlink and segregate to daughter cells during cell division, highlighting a remarkable ability of cells to organize long DNA molecules. SMC complexes promote DNA organization by loop extrusion. In most bacteria, chromosome folding initiates at dedicated start sites marked by the ParB/parS partition complexes. Whether SMC complexes recognize a specific DNA structure in the partition complex or a protein component is unclear. By replacing genes in Bacillus subtilis with orthologous sequences from Streptococcus pneumoniae, we show that the three subunits of the bacterial Smc complex together with the ParB protein form a functional module that can organize and segregate foreign chromosomes. Using chimeric proteins and chemical cross-linking, we find that ParB directly binds the Smc subunit. We map an interface to the Smc joint and the ParB CTP-binding domain. Structure prediction indicates how the ParB clamp presents DNA to the Smc complex, presumably to initiate DNA loop extrusion.
PID Serval
serval:BIB_202D0C656B23
PMID
Open Access
Oui
Date de création
2022-09-05T06:54:13.066Z
Date de création dans IRIS
2025-05-20T15:48:53Z
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Nom
36044845_BIB_202D0C656B23.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by-nc-nd/4.0
Taille
7.93 MB
Format
Adobe PDF
PID Serval
serval:BIB_202D0C656B23.P001
URN
urn:nbn:ch:serval-BIB_202D0C656B236
Somme de contrôle
(MD5):67aafded0eaa3f6467acf0cda4fc7836