Titre
Rhodamine 6G efflux for the detection of CDR1-overexpressing azole-resistant Candida albicans strains.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Maesaki, S.
Auteure/Auteur
Marichal, P.
Auteure/Auteur
Vanden Bossche, H.
Auteure/Auteur
Sanglard, D.
Auteure/Auteur
Kohno, S.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0305-7453
Statut éditorial
Publié
Date de publication
1999-07
Volume
44
Numéro
1
Première page
27
Dernière page/numéro d’article
31
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Résumé
We investigated the drug efflux mechanism in azole-resistant strains of Candida albicans using rhodamine 6G (R6G). No significant differences in R6G uptake were observed between azole-sensitive B2630 (9.02 +/- 0.02 nmol/10(8) cells) and azole-resistant B67081 (8.86 +/- 0.03 nmol/10(8) cells) strains incubated in glucose-free phosphate buffered saline. A significantly higher R6G efflux (2.0 +/- 0.21 nmol/10(8) cells) was noted in the azole-resistant strain (B67081) when glucose was added, compared with that in the sensitive strain B2630 (0.23 < or = 0.14 nmol/10(8) cells). A fluconazole-resistant strain C40 that expressed the benomyl resistance gene (CaMDR) also showed a low R6G efflux (0.16 +/- 0.06 nmol/10(8) cells) as did the sensitive strains. Accumulation of R6G in growing C. albicans cells was inversely correlated with the level of CDR1 mRNA expression. Our data also suggest that measurement of intracellular accumulation of R6G is a useful method for identification of azole-resistant strains due to CDR1-expressed drug efflux pump.
Sujets
PID Serval
serval:BIB_D64914E58A71
PMID
Open Access
Oui
Date de création
2008-01-25T13:40:28.628Z
Date de création dans IRIS
2025-05-21T02:28:40Z
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Nom
REF.pdf
Version du manuscrit
published
Taille
87.26 KB
Format
Adobe PDF
PID Serval
serval:BIB_D64914E58A71.P001
URN
urn:nbn:ch:serval-BIB_D64914E58A711
Somme de contrôle
(MD5):814d68ec6035066964df4e3f7e3cecee