Titre
Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Rumbo, M.
Auteure/Auteur
Courjault-Gautier, F.
Auteure/Auteur
Sierro, F.
Auteure/Auteur
Sirard, J.C.
Auteure/Auteur
Felley-Bosco, E.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1742-464X
Statut éditorial
Publié
Date de publication
2005
Volume
272
Numéro
2
Première page
444
Dernière page/numéro d’article
453
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
Inducible nitric oxide synthase (iNOS) functions as a homodimer. In cell extracts, iNOS molecules partition both in cytosolic and particulate fractions, indicating that iNOS exists as soluble and membrane associated forms. In this study, iNOS features were investigated in human intestinal epithelial cells stimulated with cytokines and in duodenum from mice exposed to flagellin. Our experiments indicate that iNOS is mainly associated with the particulate fraction of cell extracts. Confocal microscopy showed a preferential localization of iNOS at the apical pole of intestinal epithelial cells. In particulate fractions, iNOS dimers were more abundant than in the cytosolic fraction. Similar observations were seen in mouse duodenum samples. These results suggest that, in epithelial cells, iNOS activity is regulated by localization-dependent processes.
PID Serval
serval:BIB_34966
PMID
Open Access
Oui
Date de création
2007-11-19T11:33:17.538Z
Date de création dans IRIS
2025-05-20T20:46:04Z