Titre
Mapping of the genes coding for the two major vaccinia virus core polypeptides.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Wittek, R.
Auteure/Auteur
Richner, B.
Auteure/Auteur
Hiller, G.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0305-1048[print], 0305-1048[linking]
Statut éditorial
Publié
Date de publication
1984-06
Volume
12
Numéro
12
Première page
4835
Dernière page/numéro d’article
4848
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
We have mapped the genes coding for two major structural polypeptides of the vaccinia virus core by hybrid selection and transcriptional mapping. First, RNA was selected by hybridization to restriction fragments of the vaccinia virus genome, translated in vitro and the products were immunoprecipitated with antibodies against the two polypeptides. This approach allowed us to map the genes to the left hand end of the largest Hind III restriction fragment of 50 kilobase pairs. Second, transcriptional mapping of this region of the genome revealed the presence of the two expected RNAs. Both RNAs are transcribed from the leftward reading strand and the 5'-ends of the genes are separated by about 7.5 kilobase pairs of DNA. Thus, two genes encoding structural polypeptides with a similar location in the vaccinia virus particle are clustered at approximately 105 kilobase pairs from the left hand end of the 180 kilobase pair vaccinia virus genome.
PID Serval
serval:BIB_71D5534697D5
PMID
Date de création
2008-01-24T09:43:36.534Z
Date de création dans IRIS
2025-05-21T04:34:22Z