Titre
Assessment of the in vitro synergy of daptomycin plus linezolid against multidrug-resistant enterococci
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Journal of Global Antimicrobial Resistance
Auteur(s)
Entenza, J.M.
Auteure/Auteur
Giddey, M.
Auteure/Auteur
Vouillamoz, J.
Auteure/Auteur
Moreillon, P.
Auteure/Auteur
Mancini, S.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
2213-7165
Statut éditorial
Publié
Date de publication
2014
Volume
2
Numéro
4
Première page
306
Dernière page/numéro d’article
308
Peer-reviewed
Oui
Langue
anglais
Résumé
The widespread incidence of enterococci resistant to ampicillin, vancomycin and aminoglycosides, the first-line anti-enterococcal antibiotics, has made the treatment of severe enterococcal infections difficult and alternatives should be explored. We investigated the activity of daptomycin combined with linezolid against three Enterococcus faecalis and four Enterococcus faecium strains resistant to standard drugs used for therapy. Minimum inhibitory concentrations (MICs) were determined by the broth dilution method. Drug interactions were assessed by the checkerboard and time-kill methods. Synergy was defined by a fractional inhibitory concentration index (FICI) of ≤0.5 or a ≥2 log10 CFU/mL killing at 24 h with the combination in comparison with killing by the most active single agent. Indifference was defined by a FICI > 0.5-4.0 or a 1-2 log10 CFU/mL killing compared with the most active single agent. MICs of daptomycin were 2-4 μg/mL for E. faecalis and 2-8 μg/mL for E. faecium. MICs of linezolid were 1-2 μg/mL for all bacteria. In the checkerboard assay, five isolates showed synergism (FICI < 0.5) and two showed indifference (FICIs of 0.53 and 2). Killing studies revealed synergy of daptomycin plus linezolid against four isolates (2.2-3.7 log10 CFU/mL kill) and indifference (1.1-1.6 log10 CFU/mL kill) for the other three strains. Antagonism was not observed. In conclusion, the combination of daptomycin and linezolid had a synergistic or indifferent effect against multidrug-resistant enterococci. Additional studies are needed to explore the potential of this combination for severe enterococcal infections when first-line antibiotic combinations cannot be used.
PID Serval
serval:BIB_7FC18EAF629F
Date de création
2015-01-20T13:14:57.789Z
Date de création dans IRIS
2025-05-21T04:39:21Z
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Nom
BIB_7FC18EAF629F.P001.pdf
Version du manuscrit
published
Taille
266.45 KB
Format
Adobe PDF
PID Serval
serval:BIB_7FC18EAF629F.P001
URN
urn:nbn:ch:serval-BIB_7FC18EAF629F7
Somme de contrôle
(MD5):89db7a01369f404f9843cc0f5d5274ab