Titre
Neuronal potential of cells in early postnatal rat sciatic nerve.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Walter, I.B.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0012-1606
Statut éditorial
Publié
Date de publication
1994
Volume
161
Numéro
1
Première page
263
Dernière page/numéro d’article
273
Peer-reviewed
Oui
Langue
anglais
Résumé
A population of undifferentiated cells with neuronal potentialities were revealed in rat sciatic nerve. Explant cultures of sciatic nerve were prepared from newborn or early postnatal rat. Cultures were growth in F14 medium supplemented with 10% of fetal calf serum, incubated in a humidified 3% CO2, 97% air atmosphere. Within 2 weeks, refractile cells exhibiting the morphology of neurons were observed in all examined cultures. These cells had ovoid or multipolar refractile cells bodies with extended cytoplasmic processes. The neuronal nature of these cells was confirmed by their immunostaining with specific neuronal markers: neurofilament triplets, neuron-specific enolase, peripherin, microtubule-associated proteins, and brain spectrin. This neuronal population displayed various phenotypes. The CO2 concentration in the incubator plays an important role, since the number of differentiated neurons was lower in cultures incubated in 5% CO2. Since the sciatic nerve is devoid of nerve cell bodies in vivo, we concluded that early postnatal sciatic nerve contains crest cells with neuronal potentialities differentiating into neurons in response to the culture's environmental cues.
PID Serval
serval:BIB_9818B20DF291
PMID
Date de création
2009-03-30T08:19:38.062Z
Date de création dans IRIS
2025-05-21T01:16:31Z