Titre
Urinary analysis of four testosterone metabolites and pregnanediol by gas chromatography-combustion-isotope ratio mass spectrometry after oral administrations of testosterone.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Maître, A.
Auteure/Auteur
Saudan, C.
Auteure/Auteur
Mangin, P.
Auteure/Auteur
Saugy, M.
Auteure/Auteur
Liens vers les personnes
ISSN
0146-4760
Statut éditorial
Publié
Date de publication
2004
Volume
28
Numéro
6
Première page
426
Dernière page/numéro d’article
31
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
The most frequently used method to demonstrate testosterone abuse is the determination of the testosterone and epitestosterone concentration ratio (T/E ratio) in urine. Nevertheless, it is known that factors other than testosterone administration may increase the T/E ratio. In the last years, the determination of the carbon isotope ratio has proven to be the most promising method to help discriminate between naturally elevated T/E ratios and those reflecting T use. In this paper, an excretion study following oral administration of 40 mg testosterone undecanoate initially and 13 h later is presented. Four testosterone metabolites (androsterone, etiocholanolone, 5 alpha-androstanediol, and 5 beta-androstanediol) together with an endogenous reference (5 beta-pregnanediol) were extracted from the urines and the delta(13)C/(12)C ratio of each compound was analyzed by gas chromatography-combustion-isotope ratio mass spectrometry. The results show similar maximum delta(13)C-value variations (parts per thousand difference of delta(13)C/(12)C ratio from the isotope ratio standard) for the T metabolites and concomitant changes of the T/E ratios after administration of the first and the second dose of T. Whereas the T/E ratios as well as the androsterone, etiocholanolone and 5 alpha-androstanediol delta(13)C-values returned to the baseline 15 h after the second T administration, a decrease of the 5 beta-androstanediol delta-values could be detected for over 40 h. This suggests that measurements of 5 beta-androstanediol delta-values allow the detection of a testosterone ingestion over a longer post-administration period than other T metabolites delta(13)C-values or than the usual T/E ratio approach.
PID Serval
serval:BIB_572628EF6E09
PMID
Open Access
Oui
Date de création
2008-02-08T16:44:47.325Z
Date de création dans IRIS
2025-05-20T16:32:30Z
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Nom
REF.pdf
Version du manuscrit
published
Taille
562.88 KB
Format
Adobe PDF
PID Serval
serval:BIB_572628EF6E09.P001
URN
urn:nbn:ch:serval-BIB_572628EF6E098
Somme de contrôle
(MD5):73a968074fcc5330ea97ee6e105a84b7