Titre
Sample preparation for two-dimensional gel electrophoresis.
Type
synthèse (review)
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Shaw, M.M.
Auteure/Auteur
Riederer, B.M.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1615-9853
Statut éditorial
Publié
Date de publication
2003
Volume
3
Numéro
8
Première page
1408
Dernière page/numéro d’article
1417
Peer-reviewed
Oui
Langue
anglais
Résumé
The choice of sample preparation protocol is a critical influential factor for isoelectric focusing which in turn affects the two-dimensional gel result in terms of quality and protein species distribution. The optimal protocol varies depending on the nature of the sample for analysis and the properties of the constituent protein species (hydrophobicity, tendency to form aggregates, copy number) intended for resolution. This review explains the standard sample buffer constituents and illustrates a series of protocols for processing diverse samples for two-dimensional gel electrophoresis, including hydrophobic membrane proteins. Current methods for concentrating lower abundance proteins, by removal of high abundance proteins, are also outlined. Finally, since protein staining is becoming increasingly incorporated into the sample preparation procedure, we describe the principles and applications of current (and future) pre-electrophoretic labelling methods.
PID Serval
serval:BIB_26BA66DB37FF
PMID
Date de création
2008-01-24T13:35:05.953Z
Date de création dans IRIS
2025-05-20T16:51:31Z