Titre
1H-localized broadband 13C NMR spectroscopy of the rat brain in vivo at 9.4 T.
Type
article
Institution
Externe
Périodique
Auteur(s)
Henry, P.G.
Auteure/Auteur
Tkác, I.
Auteure/Auteur
Gruetter, R.
Auteure/Auteur
Liens vers les personnes
ISSN
0740-3194
Statut éditorial
Publié
Date de publication
2003
Volume
50
Numéro
4
Première page
684
Dernière page/numéro d’article
692
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Résumé
Localized (13)C NMR spectra were obtained from the rat brain in vivo over a broad spectral range (15-100 ppm) with minimal chemical-shift displacement error (<10%) using semi-adiabatic distortionless enhancement by polarization transfer (DEPT) combined with (1)H localization. A new gradient dephasing scheme was employed to eliminate unwanted coherences generated by DEPT when using surface coils with highly inhomogeneous B(1) fields. Excellent sensitivity was evident from the simultaneous detection of natural abundance signals for N-acetylaspartate, myo-inositol, and glutamate in the rat brain in vivo at 9.4 T. After infusion of (13)C-labeled glucose, up to 18 (13)C resonances were simultaneously measured in the rat brain, including glutamate C2, C3, C4, glutamine C2, C3, C4, aspartate C2, C3, glucose C1, C6, N-acetyl-aspartate C2, C3, C6, as well as GABA C2, lactate C3, and alanine C3. (13)C-(13)C multiplets corresponding to multiply labeled compounds were clearly observed, suggesting that extensive isotopomer analysis is possible in vivo. This unprecedented amount of information will be useful for metabolic modeling studies aimed at understanding brain energy metabolism and neurotransmission in the rodent brain.
PID Serval
serval:BIB_E3EBE3D93FED
PMID
Open Access
Oui
Date de création
2010-08-04T14:28:11.545Z
Date de création dans IRIS
2025-05-21T06:37:56Z