Titre
Targeting of SCG10 to the area of the Golgi complex is mediated by its NH2-terminal region.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Di Paolo, G.
Auteure/Auteur
Lutjens, R.
Auteure/Auteur
Pellier, V.
Auteure/Auteur
Stimpson, S.A.
Auteure/Auteur
Beuchat, M.H.
Auteure/Auteur
Catsicas, S.
Auteure/Auteur
Grenningloh, G.
Auteure/Auteur
Liens vers les unités
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
1997
Volume
272
Numéro
8
Première page
5175
Dernière page/numéro d’article
5182
Langue
anglais
Résumé
SCG10 is a neuronal growth-associated protein that is concentrated in the growth cones of developing neurons. SCG10 shows a high degree of sequence homology to the ubiquitous phosphoprotein stathmin, which has been recently identified as a factor that destabilizes microtubules by increasing their catastrophe rate. Whereas stathmin is a soluble cytosolic protein, SCG10 is membrane-associated, indicating that the protein acts in a distinct subcellular compartment. Identifying the precise intracellular distribution of SCG10 as well as the mechanisms responsible for its specific targeting will contribute to elucidating its function. The main structural feature distinguishing the two proteins is that SCG10 contains an NH2-terminal extension of 34 amino acids. In this study, we have examined the intracellular distribution of SCG10 in PC12 cells and in transfected COS-7 cells and the role of the NH2-terminal domain in membrane-binding and intracellular targeting. SCG10 was found to be localized to the Golgi complex region. We show that the NH2-terminal region (residues 1-34) was necessary for membrane targeting and Golgi localization. Fusion proteins consisting of the NH2-terminal 34 amino acids of SCG10 and the related protein stathmin or the unrelated protein, beta-galactosidase, accumulated in the Golgi, demonstrating that this sequence was sufficient for Golgi localization. Biosynthetic labeling of transfected COS-7 cells with [3H]palmitic acid revealed that two cysteine residues contained within the NH2-terminal domain were sites of palmitoylation.
PID Serval
serval:BIB_327
PMID
Open Access
Oui
Date de création
2007-11-19T11:31:48.055Z
Date de création dans IRIS
2025-05-20T13:23:34Z