Titre
Reduction-Sensitive Protein Nanogels Enhance Uptake of Model and Tumor Lysate Antigens In Vitro by Mouse- and Human-Derived Dendritic Cells.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Berti, C.
Auteure/Auteur
Boarino, A.
Auteure/Auteur
Graciotti, M.
Auteure/Auteur
Bader, LPE
Auteure/Auteur
Kandalaft, L.E.
Auteure/Auteur
Klok, H.A.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
2576-6422
Statut éditorial
Publié
Date de publication
2021-12-20
Volume
4
Numéro
12
Première page
8291
Dernière page/numéro d’article
8300
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Peptides and proteins represent an emerging class of powerful therapeutics. Peptide and protein nanogels are attractive carriers for the transport and delivery of biologically active peptides and proteins because they allow essentially quantitative encapsulation of these biologics. One interesting field of use of peptide and protein nanogels is the transport of antigens and adjuvants in cancer immunotherapy. This study demonstrates the use of reduction-sensitive protein nanogels for the delivery of ovalbumin and oxidized tumor lysate-based antigens to mouse and human-donor-derived dendritic cells. Challenging mouse-derived and human dendritic cells with reduction-sensitive ovalbumin nanogels was found to significantly enhance antigen uptake as compared to the use of the corresponding free protein antigen. The experiments with mouse-derived dendritic cells further showed that the administration of ovalbumin in the form of reduction-sensitive nanogels enhanced dendritic cell maturation as well as the presentation of the SIINFEKL epitope as compared to experiments that use free ovalbumin. In addition to ovalbumin as a model antigen, the feasibility of reduction-sensitive nanogels was also demonstrated for the delivery of oxidized, whole tumor lysate-based cancer antigens. In experiments with dendritic cells harvested from human donors, dendritic cell uptake of the oxidized tumor lysate antigen was significantly enhanced in experiments that used oxidized tumor lysate nanogels as compared to the free antigen.
PID Serval
serval:BIB_0D82E169FC14
PMID
Open Access
Oui
Date de création
2022-01-17T10:10:00.845Z
Date de création dans IRIS
2025-05-20T14:23:17Z
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Nom
acsabm.1c00828.pdf
Version du manuscrit
preprint
Taille
4.21 MB
Format
Adobe PDF
PID Serval
serval:BIB_0D82E169FC14.P001
Somme de contrôle
(MD5):b8c2a8f76a2cb409cd9f6d58825a4f6a