Titre
The estrogen-responsive B box protein: a novel regulator of keratinocyte differentiation.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Beer, H.D.
Auteure/Auteur
Munding, C.
Auteure/Auteur
Dubois, N.
Auteure/Auteur
Mamie, C.
Auteure/Auteur
Hohl, D.
Auteure/Auteur
Werner, S.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
2002-06
Volume
277
Numéro
23
Première page
20740
Dernière page/numéro d’article
20749
Peer-reviewed
Oui
Langue
anglais
Résumé
Keratinocyte growth factor (KGF) regulates proliferation, differentiation, migration, and survival of different types of epithelial cells, including keratinocytes of the skin. To gain insight into the mechanisms underlying these multiple functions, we searched for KGF- regulated genes in keratinocytes. Using the differential display reverse transcriptase-PCR technology, we identified the gene encoding the estrogen-responsive B box protein (EBBP) which has as yet not been functionally characterized. The full-length murine and human EBBP cDNAs were cloned and fully sequenced. They were shown to encode 75-kDa proteins, which are mainly localized in the cytoplasm of keratinocytes in vitro and in vivo. In vivo, EBBP was found at high levels in the KGF- and epidermal growth factor-responsive basal keratinocytes of human skin, but the expression was down-regulated in the hyperthickened epithelium of skin wounds. Stable overexpression of EBBP in HaCaT keratinocytes did not affect the proliferation rate of the transfected cells, but enhanced the early differentiation process. These results suggest that the presence of EBBP in basal keratinocytes is important for the differentiation capacity of these cells, and that down-regulation of EBBP expression in a hyperproliferative epithelium is required to maintain the cells in a non-differentiated stage.
Sujets
PID Serval
serval:BIB_A9EF006086E4
PMID
Open Access
Oui
Date de création
2008-01-25T15:35:51.503Z
Date de création dans IRIS
2025-05-21T02:58:17Z