Titre
Detecting natural abundance carbon signal of NAA metabolite within 12-cm3 localized volume of human brain using 1H-[13C] NMR spectroscopy.
Type
article
Institution
Externe
Périodique
Auteur(s)
Chen, W.
Auteure/Auteur
Adriany, G.
Auteure/Auteur
Zhu, X.H.
Auteure/Auteur
Gruetter, R.
Auteure/Auteur
Ugurbil, K.
Auteure/Auteur
Liens vers les personnes
ISSN
0740-3194
Statut éditorial
Publié
Date de publication
1998
Volume
40
Numéro
2
Première page
180
Dernière page/numéro d’article
184
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Résumé
NMR spectroscopy has been applied extensively to study metabolism noninvasively in the human brain and other tissues. However, it usually suffers from poor signal-to-noise ratio due to low NMR sensitivity and low metabolite concentrations. In this study, the technique of proton-observe-carbon-edited (POCE) NMR spectroscopy combined with a single-shot localization sequence was used to detect the natural abundance carbon signal of the amino acid N-acetyl aspartate from a 12-cm3 localized volume in the occipital lobe of humans at 4 T. The results suggest that NMR spectroscopy is sensitive enough to detect signals from low concentration metabolites (< 60 nmol/g) from small volumes in the human brain within several minutes of data acquisition. This reveals that in vivo NMR spectroscopy is a promising technique for detecting small metabolite changes and low traces of 13C isotopic labeling for dynamic metabolism studies aimed at investigating physiological and pathological questions.
PID Serval
serval:BIB_E7C70A2D7E01
PMID
Date de création
2010-08-04T14:28:19.427Z
Date de création dans IRIS
2025-05-21T06:01:43Z