Titre
A conserved domain and membrane targeting of Nef from HIV and SIV are required for association with a cellular serine kinase activity
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Sawai, E. T.
Auteure/Auteur
Baur, A. S.
Auteure/Auteur
Peterlin, B. M.
Auteure/Auteur
Levy, J. A.
Auteure/Auteur
Cheng-Mayer, C.
Auteure/Auteur
Liens vers les unités
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
1995
Volume
270
Numéro
25
Première page
15307
Dernière page/numéro d’article
15314
Notes
PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S
Résumé
Among the primate lentiviruses (human immunodeficiency virus (HIV) -1, HIV-2, and simian immunodeficiency virus (SIV), the nef gene is highly conserved and encodes a myristylated protein of approximately 27 kDa (HIV-1) or approximately 34 kDa (HIV-2, SIV). Previously, we found Nef expressed either as a CD8-Nef fusion protein or as a native protein in virally infected T cell lines associates with a cellular serine kinase. This kinase activity phosphorylated two proteins of 62 and 72 kDa that coimmunoprecipitate with Nef in in vitro kinase assays. Using transient expression, various Nef alleles and mutants have been analyzed for association with the cellular kinase activity. The ability of Nef to associate with the kinase activity is conserved among several alleles of HIV-1 as well as SIVmac239 and is observed in non-lymphoid cell lines of simian and murine origins. Two separate regions of HIV-1SF2 Nef are critical for the associated kinase activity. One domain overlaps with a central highly conserved region found in all primate lentivirus nef genes and has been provisionally mapped to amino acids 45-127. Because membrane localization of Nef is important for the associated cellular kinase activity, the second domain represents a membrane targeting signal. Moreover, point mutations within the central region that abrogate the Nef-associated kinase activity in HIV-1SF2 Nef have the same effect when introduced into SIVmac239open Nef
Sujets
PID Serval
serval:BIB_6A2FAA02A825
PMID
Open Access
Oui
Date de création
2008-01-29T17:34:37.736Z
Date de création dans IRIS
2025-05-20T21:23:19Z