Titre
Development of a large-scale rapid LAMP diagnostic testing platform for pandemic preparedness and outbreak response.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
van Beuningen, R.
Auteure/Auteur
Jim, K.K.
Auteure/Auteur
Boot, M.
Auteure/Auteur
Ossendrijver, M.
Auteure/Auteur
Keijser, BJF
Auteure/Auteur
van de Bovenkamp, JHB
Auteure/Auteur
Melchers, WJG
Auteure/Auteur
Kievits, T.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
2396-8923
Statut éditorial
Publié
Date de publication
2024
Volume
9
Numéro
1
Première page
bpae090
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
The coronavirus disease 2019 (COVID-19) pandemic underscored the necessity for rapid and efficient diagnostic testing to mitigate outbreaks and control disease transmission. While real-time reverse transcriptase quantitative PCR (RT-qPCR) has been the gold standard due to its high sensitivity and specificity, its logistical complexities and extended turnaround times highlighted the need for alternative molecular methods and non-standard equipment and consumables not subject to supply chain pressure. Loop-mediated isothermal amplification (LAMP) offers several advantages over RT-qPCR, including faster processing time, assay flexibility and cost-effectiveness. During the pandemic, LAMP was successfully demonstrated as a viable alternative to RT-qPCR for SARS-Related Coronavirus 2 detection. However, due to a 100 to 1,000-fold increase in testing volumes, there was an imminent need for automating and scaling up existing LAMP testing workflows leveraging a robotic infrastructure, while retaining analytical performance and cost-effectiveness. In 2020, the Foundation TOMi started the "TOMi corona initiative" to develop and validate a high-throughput, end-to-end, automated, scalable single-step RNA purification, and LAMP-based COVID-19 testing system called SMART-LAMP (Scalable Molecular Automation for Rapid Testing using LAMP) that can process up to 40,000 samples per day using existing laboratory equipment infrastructure with sensitivity comparable to RT-qPCR. This system provides a rapid and scalable diagnostic solution for future pandemics, capable of processing over 40,000 samples per day. In addition, the system is designed to minimize consumable costs and reduces the overall use of plastics to align with increasingly strict sustainability goals that will be imposed over the coming years. Importantly, this system and public-private partnerships in the TOMi corona initiative has the potential to serve as a baseline to enhance pandemic preparedness and response capabilities.
PID Serval
serval:BIB_CCD47FC69EA4
PMID
Open Access
Oui
Date de création
2024-12-16T15:25:26.746Z
Date de création dans IRIS
2025-05-21T03:36:20Z
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Nom
39664603_BIB_CCD47FC69EA4.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by/4.0
Taille
2.08 MB
Format
Adobe PDF
PID Serval
serval:BIB_CCD47FC69EA4.P001
URN
urn:nbn:ch:serval-BIB_CCD47FC69EA45
Somme de contrôle
(MD5):b28e86bc21fe30c7be80344905540274