Titre
Stress‐ and pathway‐specific impacts of impaired jasmonoyl‐isoleucine (JA‐Ile) catabolism on defense signalling and biotic stress resistance
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Marquis, Valentin
Auteure/Auteur
Smirnova, Ekaterina
Auteure/Auteur
Poirier, Laure
Auteure/Auteur
Zumsteg, Julie
Auteure/Auteur
Schweizer, Fabian
Auteure/Auteur
Reymond, Philippe
Auteure/Auteur
Heitz, Thierry
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
Statut éditorial
Publié
Date de publication
2020-06
Volume
43
Numéro
6
Première page
1558
Dernière page/numéro d’article
1570
Langue
anglais
Résumé
Jasmonate synthesis and signalling are essential for plant defense upregulation upon herbivore or microbial attacks. Stress-induced accumulation of jasmonoyl-isoleucine (JA-Ile), the bioactive hormonal form triggering transcriptional changes, is dynamic and transient because of the existence of potent removal mechanisms. Two JA-Ile turnover pathways operate in Arabidopsis, consisting in cytochrome P450 (CYP94)mediated oxidation and deconjugation by the amidohydrolases IAR3/ILL6. Understanding their impacts was previously blurred by gene redundancy and compensation mechanisms. Here we address the consequences of blocking these pathways on jasmonate homeostasis and defenses in double-2ah, triple-3cyp mutants, and a quintuple-5ko line deficient in all known JA-Ile-degrading activities. These lines reacted differently to either mechanical wounding/insect attack or fungal infection. Both pathways contributed additively to JA-Ile removal upon wounding, but their impairement had opposite impacts on insect larvae feeding. By contrast, only the ah pathway was essential for JA-Ile turnover upon infection by Botrytis, yet only 3cyp was more fungus-resistant. Despite building-up extreme JA-Ile levels, 5ko displayed near-wild-type resistance in both bioassays. Molecular analysis indicated that restrained JA-Ile catabolism resulted in enhanced defense/resistance only when genes encoding negative regulators were not simultaneously overstimulated. This occurred in discrete stress- and pathway-specific combinations, providing a framework for future defense-enhancing strategies.
PID Serval
serval:BIB_A106CC37CE3F
Date de création
2020-06-02T12:41:20.917Z
Date de création dans IRIS
2025-05-20T21:41:04Z
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Nom
Marquis_et_al-author manuscript.pdf
Version du manuscrit
preprint
Taille
2.33 MB
Format
Adobe PDF
PID Serval
serval:BIB_A106CC37CE3F.P001
URN
urn:nbn:ch:serval-BIB_A106CC37CE3F3
Somme de contrôle
(MD5):d1ba34f4b876bd2d9a50bec926bc186d