Titre
CYK-4 functions independently of its centralspindlin partner ZEN-4 to cellularize oocytes in germline syncytia.
Type
article
Institution
Externe
Périodique
Auteur(s)
Lee, K.Y.
Auteure/Auteur
Green, R.A.
Auteure/Auteur
Gutierrez, E.
Auteure/Auteur
Gomez-Cavazos, J.S.
Auteure/Auteur
Kolotuev, I.
Auteure/Auteur
Wang, S.
Auteure/Auteur
Desai, A.
Auteure/Auteur
Groisman, A.
Auteure/Auteur
Oegema, K.
Auteure/Auteur
Liens vers les personnes
ISSN
2050-084X
Statut éditorial
Publié
Date de publication
2018-07-10
Volume
7
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
Publication Status: epublish
Publication Status: epublish
Résumé
Throughout metazoans, germ cells undergo incomplete cytokinesis to form syncytia connected by intercellular bridges. Gamete formation ultimately requires bridge closure, yet how bridges are reactivated to close is not known. The most conserved bridge component is centralspindlin, a complex of the Rho family GTPase-activating protein (GAP) CYK-4/MgcRacGAP and the microtubule motor ZEN-4/kinesin-6. Here, we show that oocyte production by the syncytial Caenorhabditis elegans germline requires CYK-4 but not ZEN-4, which contrasts with cytokinesis, where both are essential. Longitudinal imaging after conditional inactivation revealed that CYK-4 activity is important for oocyte cellularization, but not for the cytokinesis-like events that generate syncytial compartments. CYK-4's lipid-binding C1 domain and the GTPase-binding interface of its GAP domain were both required to target CYK-4 to intercellular bridges and to cellularize oocytes. These results suggest that the conserved C1-GAP region of CYK-4 constitutes a targeting module required for closure of intercellular bridges in germline syncytia.
Sujets
PID Serval
serval:BIB_613C15D527AF
PMID
Open Access
Oui
Date de création
2021-10-01T07:39:36.928Z
Date de création dans IRIS
2025-05-20T20:58:58Z
Fichier(s)![Vignette d'image]()
En cours de chargement...
Nom
elife-36919.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by/4.0
Taille
10.85 MB
Format
Adobe PDF
PID Serval
serval:BIB_613C15D527AF.P001
Somme de contrôle
(MD5):4b42a7f52300899f0039dcb5e2ee542d