Titre
A biosensor for measuring NAD+ levels at the point of care
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Yu, Q.
Auteure/Auteur
Pourmandi, N.
Auteure/Auteur
Xue, L.
Auteure/Auteur
Gondrand, C.
Auteure/Auteur
Fabritz, S.
Auteure/Auteur
Bardy, D.
Auteure/Auteur
Patiny, L.
Auteure/Auteur
Katsyuba, E.
Auteure/Auteur
Auwerx, J.
Auteure/Auteur
Johnsson, K.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
2522-5812
Statut éditorial
Publié
Date de publication
2019-12
Volume
1
Numéro
12
Première page
1219
Dernière page/numéro d’article
1225
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
The cellular level of nicotinamide adenine dinucleotide (NAD <sup>+</sup> ), through its different functions, affects cellular metabolism and signalling <sup>1-3</sup> . A decrease in the NAD <sup>+</sup> content has been associated with various pathologies and physiological aging <sup>4,5</sup> , while strategies to boost cellular NAD <sup>+</sup> levels have been shown to be effective against age-related diseases in many animal models <sup>6</sup> . The link between decreased NAD <sup>+</sup> levels and numerous pathologies and physiological aging has triggered the need for a simple quantification method for NAD <sup>+</sup> , ideally applicable at the point of care. Here, we introduce a bioluminescent biosensor for the rapid quantification of NAD <sup>+</sup> levels in biological samples, which can be used either in laboratories or at the point of care. The biosensor is a semisynthetic, light-emitting sensor protein that changes the colour of emitted light from blue to red on binding of NAD <sup>+</sup> . This NAD <sup>+</sup> -dependent colour change enables the use of the biosensor in paper-based assays in which NAD <sup>+</sup> is quantified by measuring the colour of the emitted light by using either a simple digital camera or a plate reader. We used the approach to quantify NAD <sup>+</sup> levels in cell culture, tissue and blood samples, yielding results that agreed with those from standard testing methods. The same biosensor furthermore allows the quantification of NAD <sup>+</sup> -dependent enzymatic activities in blood samples, thus expanding its utility as a tool for point-of-care diagnostics.
PID Serval
serval:BIB_F96DE6C05AB9
PMID
Date de création
2020-07-24T11:46:56.138Z
Date de création dans IRIS
2025-05-21T05:21:55Z