Titre
Detection of JC virus-specific cytotoxic T lymphocytes in healthy individuals.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Du Pasquier, R.A.
Auteure/Auteur
Schmitz, J.E.
Auteure/Auteur
Jean-Jacques, J.
Auteure/Auteur
Zheng, Y.
Auteure/Auteur
Gordon, J.
Auteure/Auteur
Khalili, K.
Auteure/Auteur
Letvin, N.L.
Auteure/Auteur
Koralnik, I.J.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0022-538X[print], 0022-538X[linking]
Statut éditorial
Publié
Date de publication
2004
Volume
78
Numéro
18
Première page
10206
Dernière page/numéro d’article
10210
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Publication Status: ppublish
Résumé
The polyomavirus JC (JCV) infects 85% of healthy individuals, and its reactivation in a limited number of immunosuppressed people causes progressive multifocal leukoencephalopathy (PML), a severe demyelinating disease of the central nervous system. We hypothesized that JCV-specific cytotoxic T lymphocytes (CTLs) might control JCV replication in healthy individuals, blocking the evolution of PML. Using 51Cr release and tetramer staining assays, we show that 8 of 11 HLA-A*0201+ healthy subjects (73%) harbor detectable JCV-specific CD8+ CTLs that recognize one or two epitopes of JCV VP1 protein, the HLA-A*0201-restricted VP1p36 and VPp1100 epitopes. We determined that the frequency of JCV VP1 epitope-specific CTLs varied from less than 1/100,000 to 1/2,494 peripheral blood mononuclear cells. More individuals had JCV VP1-specific than cytomegalovirus-specific CTLs (8 of 11 subjects [73%] versus 2 of 10 subjects [20%], respectively). These results show that a CD8+-T-cell response against JCV is commonly found in immunocompetent people and suggest that these cells might protect against the development of PML.
Sujets
PID Serval
serval:BIB_818367D60578
PMID
Open Access
Oui
Date de création
2008-01-25T13:56:18.367Z
Date de création dans IRIS
2025-05-21T02:09:00Z