Titre
Neuronal nitric oxide synthase does not contribute to the modulation of pulmonary vascular tone in fetal lambs with congenital diaphragmatic hernia (nNOS in CDH lambs).
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
de Buys Roessingh, A.S.
Auteure/Auteur
de Lagausie, P.
Auteure/Auteur
Ebrahimian, T.
Auteure/Auteur
Ibrahima, T.
Auteure/Auteur
Duong-Quy, S.
Auteure/Auteur
Schneider, J.C.
Auteure/Auteur
Huang, X.L.
Auteure/Auteur
Mercier, J.C.
Auteure/Auteur
Aigrain, Y.
Auteure/Auteur
Boulanger, C.
Auteure/Auteur
Dinh-Xuan, A.T.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1099-0496
Statut éditorial
Publié
Date de publication
2008
Volume
43
Numéro
4
Première page
313
Dernière page/numéro d’article
321
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
AIM: The aim of this study was to determine the presence of the neuronal nitric oxide synthase (nNOS) in near full-term lambs with congenital diaphragmatic hernia (CDH) and its role in the modulation of pulmonary vascular basal tone. METHODS: We surgically created diaphragmatic hernia on the 85th day of gestation. On the 135th, catheters were used to measure pulmonary pressure and blood flow. We tested the effects of 7-nitroindazole (7-NINA), a specific nNOS antagonist and of N-nitro-L-arginine (L-NNA), a nonspecific nitric oxide synthase antagonist. In vitro, we tested the effects of the same drugs on isolated pulmonary vessels. The presence of nNOS protein in the lungs was detected by Western blot analysis. RESULTS: Neither 7-NINA nor L-NNA modified pulmonary vascular basal tone in vivo. After L-NNA injection, acetylcholine (ACh) did not decrease significantly pulmonary vascular resistance (PVR). In vitro, L-NNA increased the cholinergic contractile-response elicited by electric field stimulation (EFS) of vascular rings from lambs with diaphragmatic hernia. CONCLUSION: We conclude that nNOS protein is present in the lungs and pulmonary artery of near full-term lamb fetuses with diaphragmatic hernia, but that it does not contribute to the reduction of pulmonary vascular tone at birth
Sujets
PID Serval
serval:BIB_A773EB7D32C6
PMID
Date de création
2009-01-29T21:13:17.141Z
Date de création dans IRIS
2025-05-20T23:41:03Z