Titre
Bcl-2 decreases the free Ca2+ concentration within the endoplasmic reticulum
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Auteur(s)
Foyouzi-Youssefi, R.
Auteure/Auteur
Arnaudeau, S.
Auteure/Auteur
Borner, C.
Auteure/Auteur
Kelley, W. L.
Auteure/Auteur
Tschopp, J.
Auteure/Auteur
Lew, D. P.
Auteure/Auteur
Demaurex, N.
Auteure/Auteur
Krause, K. H.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0027-8424
Statut éditorial
Publié
Date de publication
2000-05
Volume
97
Numéro
11
Première page
5723
Dernière page/numéro d’article
8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May 23
Research Support, Non-U.S. Gov't --- Old month value: May 23
Résumé
The antiapoptotic protein Bcl-2 localizes not only to mitochondria but also to the endoplasmic reticulum (ER). However, the function of Bcl-2 at the level of the ER is poorly understood. In this study, we have investigated the effects of Bcl-2 expression on Ca(2+) storage and release by the ER. The expression of Bcl-2 decreased the amount of Ca(2+) that could be released from intracellular stores, regardless of the mode of store depletion, the cell type, or the species from which Bcl-2 was derived. Bcl-2 also decreased cellular Ca(2+) store content in the presence of mitochondrial inhibitors, suggesting that its effects were not mediated through mitochondrial Ca(2+) uptake. Direct measurements with ER-targeted Ca(2+)-sensitive fluorescent "cameleon" proteins revealed that Bcl-2 decreased the free Ca(2+) concentration within the lumen of the ER, [Ca(2+)](ER). Analysis of the kinetics of Ca(2+) store depletion in response to the Ca(2+)-ATPase inhibitor thapsigargin revealed that Bcl-2 increased the permeability of the ER membrane. These results suggest that Bcl-2 decreases the free Ca(2+) concentration within the ER lumen by increasing the Ca(2+) permeability of the ER membrane. The increased ER Ca(2+) permeability conferred by Bcl-2 would be compatible with an ion channel function of Bcl-2 at the level of the ER membrane.
Sujets
PID Serval
serval:BIB_9FA3AAA50649
PMID
Open Access
Oui
Date de création
2008-01-24T14:19:22.781Z
Date de création dans IRIS
2025-05-20T23:41:31Z