Titre
Electrospray micromixer chip for on-line derivatization and kinetic studies.
Type
article
Institution
Externe
Périodique
Auteur(s)
Abonnenc, M.
Auteure/Auteur
Dayon, L.
Auteure/Auteur
Perruche, B.
Auteure/Auteur
Lion, N.
Auteure/Auteur
Girault, H.H.
Auteure/Auteur
Liens vers les personnes
ISSN
1520-6882
Statut éditorial
Publié
Date de publication
2008
Volume
80
Numéro
9
Première page
3372
Dernière page/numéro d’article
3378
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
An electrospray microchip for mass spectrometry comprising an integrated passive mixer to carry out on-chip chemical derivatizations is described. The microchip fabricated using UV-photoablation is composed of two microchannels linked together by a liquid junction. Downstream of this liquid junction, a mixing unit made of parallel oblique grooves is integrated to the microchannel in order to create flow perturbations. Several mixer designs are evaluated. The mixer efficiency is investigated both by fluorescence study and mass spectrometric monitoring of the tagging reaction of cysteinyl peptides with 1,4-benzoquinone. The comparisons with a microchip without a mixing unit and a kinetic model are used to assess the efficiency of the mixer showing tagging kinetics close to that of bulk reactions in an ideally mixed reactor. As an ultimate application, the electrospray micromixer is implemented in a LC-MS workflow. On-line derivatization of albumin tryptic peptides after a reversed-phase separation and counting of their cysteines drastically enhance the protein identification.
Sujets
PID Serval
serval:BIB_061E74CFEF88
PMID
Date de création
2014-11-09T21:44:16.959Z
Date de création dans IRIS
2025-05-20T16:09:18Z