Titre
CYK-4: A Rho family gtpase activating protein (GAP) required for central spindle formation and cytokinesis.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Jantsch-Plunger, V.
Auteure/Auteur
Gönczy, P.
Auteure/Auteur
Romano, A.
Auteure/Auteur
Schnabel, H.
Auteure/Auteur
Hamill, D.
Auteure/Auteur
Schnabel, R.
Auteure/Auteur
Hyman, A.A.
Auteure/Auteur
Glotzer, M.
Auteure/Auteur
Liens vers les unités
ISSN
0021-9525
Statut éditorial
Publié
Date de publication
2000
Volume
149
Numéro
7
Première page
1391
Dernière page/numéro d’article
1404
Langue
anglais
Notes
Publication types: Journal Article
Résumé
During cytokinesis of animal cells, the mitotic spindle plays at least two roles. Initially, the spindle positions the contractile ring. Subsequently, the central spindle, which is composed of microtubule bundles that form during anaphase, promotes a late step in cytokinesis. How the central spindle assembles and functions in cytokinesis is poorly understood. The cyk-4 gene has been identified by genetic analysis in Caenorhabditis elegans. Embryos from cyk-4(t1689ts) mutant hermaphrodites initiate, but fail to complete, cytokinesis. These embryos also fail to assemble the central spindle. We show that the cyk-4 gene encodes a GTPase activating protein (GAP) for Rho family GTPases. CYK-4 activates GTP hydrolysis by RhoA, Rac1, and Cdc42 in vitro. RNA-mediated interference of RhoA, Rac1, and Cdc42 indicates that only RhoA is essential for cytokinesis and, thus, RhoA is the likely target of CYK-4 GAP activity for cytokinesis. CYK-4 and a CYK-4:GFP fusion protein localize to the central spindle and persist at cell division remnants. CYK-4 localization is dependent on the kinesin-like protein ZEN-4/CeMKLP1 and vice versa. These data suggest that CYK-4 and ZEN-4/CeMKLP1 cooperate in central spindle assembly. Central spindle localization of CYK-4 could accelerate GTP hydrolysis by RhoA, thereby allowing contractile ring disassembly and completion of cytokinesis.
Sujets
PID Serval
serval:BIB_17100
PMID
Open Access
Oui
Date de création
2007-11-19T11:10:39.414Z
Date de création dans IRIS
2025-05-20T14:44:19Z
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Nom
10871280_BIB_17100.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by-nc-sa/4.0
Taille
644.08 KB
Format
Adobe PDF
PID Serval
serval:BIB_17100.P001
URN
urn:nbn:ch:serval-BIB_171005
Somme de contrôle
(MD5):42ab58d63f0432b3cb392d1d0888eb86