Titre
Deep thiopental anesthesia alters steady-state glucose homeostasis but not the neurochemical profile of rat cortex.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Lei, Hongxia
Auteure/Auteur
Duarte Joao, M. N.
Auteure/Auteur
Mlynarik, Vladimir
Auteure/Auteur
Python, Agathe
Auteure/Auteur
Gruetter, Rolf
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
1097-4547[electronic]
Statut éditorial
Publié
Date de publication
2010
Volume
88
Numéro
2
Première page
413
Dernière page/numéro d’article
419
Langue
anglais
Notes
BIB_882587EB2F63
Résumé
Barbiturates are regularly used as an anesthetic for animal experimentation and clinical procedures and are frequently provided with solubilizing compounds, such as ethanol and propylene glycol, which have been reported to affect brain function and, in the case of (1)H NMR experiments, originate undesired resonances in spectra affecting the quantification. As an alternative, thiopental can be administrated without any solubilizing agents. The aim of the study was to investigate the effect of deep thiopental anesthesia on the neurochemical profile consisting of 19 metabolites and on glucose transport kinetics in vivo in rat cortex compared with alpha-chloralose using localized (1)H NMR spectroscopy. Thiopental was devoid of effects on the neurochemical profile, except for the elevated glucose at a given plasma glucose level resulting from thiopental-induced depression of glucose consumption at isoelectrical condition. Over the entire range of plasma glucose levels, steady-state glucose concentrations were increased on average by 48% +/- 8%, implying that an effect of deep thiopental anesthesia on the transport rate relative to cerebral glucose consumption ratio was increased by 47% +/- 8% compared with light alpha-chloralose-anesthetized rats. We conclude that the thiopental-induced isoelectrical condition in rat cortex significantly affected glucose contents by depressing brain metabolism, which remained substantial at isoelectricity.
PID Serval
serval:BIB_69907B4B4BED
PMID
Date de création
2011-06-15T10:01:53.601Z
Date de création dans IRIS
2025-05-20T19:00:24Z