Titre
Hyperammonemia: regulation of argininosuccinate synthetase and argininosuccinate lyase genes in aggregating cell cultures of fetal rat brain.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Braissant, O.
Auteure/Auteur
Honegger, P.
Auteure/Auteur
Loup, M.
Auteure/Auteur
Iwase, K.
Auteure/Auteur
Takiguchi, M.
Auteure/Auteur
Bachmann, C.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0304-3940
Statut éditorial
Publié
Date de publication
1999
Volume
266
Numéro
2
Première page
89
Dernière page/numéro d’article
92
Langue
anglais
Résumé
Hyperammonemia in the brain leads to poorly understood alterations of nitric oxide (NO) synthesis. Arginine, the substrate of nitric oxide synthases, might be recycled from the citrulline produced with NO by argininosuccinate synthetase (AS) and argininosuccinate lyase (AL). The regulation of AS and AL genes during hyperammonemia is unknown in the brain. We used brain cell aggregates cultured from dissociated telencephalic cortex of rat embryos to analyze the regulation of AS and AL genes in hyperammonemia. Using RNase protection assay and non-radioactive in situ hybridization on aggregate cryosections, we show that both AS and AL genes are induced in astrocytes but not in neurons of aggregates exposed to 5 mM NH4Cl. Our work suggests that the hyperammonemic brain might increase its recycling of citrulline to arginine.
PID Serval
serval:BIB_E9BFC4224715
PMID
Open Access
Oui
Date de création
2008-04-25T09:31:41.388Z
Date de création dans IRIS
2025-05-21T07:08:50Z
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Nom
BIB_E9BFC4224715.P001.pdf
Version du manuscrit
preprint
Taille
406.32 KB
Format
Adobe PDF
PID Serval
serval:BIB_E9BFC4224715.P001
URN
urn:nbn:ch:serval-BIB_E9BFC42247157
Somme de contrôle
(MD5):abdb09c677618b8d928438f711ea1faf