Titre
Histomorphological Phenotyping of the Adult Mouse Brain.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Current protocols in mouse biology
Auteur(s)
Mikhaleva, A.
Auteure/Auteur
Kannan, M.
Auteure/Auteur
Wagner, C.
Auteure/Auteur
Yalcin, B.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
2161-2617
Statut éditorial
Publié
Date de publication
2016-09-01
Volume
6
Numéro
3
Première page
307
Dernière page/numéro d’article
332
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
This article describes a series of standard operating procedures for morphological phenotyping of the mouse brain using basic histology. Many histological studies of the mouse brain use qualitative approaches based on what the human eye can detect. Consequently, some phenotypic information may be missed. Here we describe a quantitative approach for the assessment of brain morphology that is simple and robust. A total of 78 measurements are made throughout the brain at specific and well-defined regions, including the cortex, the hippocampus, and the cerebellum. Experimental design and timeline considerations, including strain background effects, the importance of sectioning quality, measurement variability, and efforts to correct human errors are discussed. © 2016 by John Wiley & Sons, Inc.
PID Serval
serval:BIB_3410E4929B2B
PMID
Date de création
2016-09-16T19:45:24.430Z
Date de création dans IRIS
2025-05-20T18:18:59Z