Titre
Fetal RHD Screening in RH1 Negative Pregnant Women: Experience in Switzerland.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Schimanski, B.
Auteure/Auteur
Kräuchi, R.
Auteure/Auteur
Stettler, J.
Auteure/Auteur
Lejon Crottet, S.
Auteure/Auteur
Niederhauser, C.
Auteure/Auteur
Clausen, F.B.
Auteure/Auteur
Fontana, S.
Auteure/Auteur
Hodel, M.
Auteure/Auteur
Amylidi-Mohr, S.
Auteure/Auteur
Raio, L.
Auteure/Auteur
Abbal, C.
Auteure/Auteur
Henny, C.
Auteure/Auteur
Liens vers les unités
ISSN
2227-9059
Statut éditorial
Publié
Date de publication
2023-09-27
Volume
11
Numéro
10
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
RH1 incompatibility between mother and fetus can cause hemolytic disease of the fetus and newborn. In Switzerland, fetal RHD genotyping from maternal blood has been recommended from gestational age 18 onwards since the year 2020. This facilitates tailored administration of RH immunoglobulin (RHIG) only to RH1 negative women carrying a RH1 positive fetus. Data from 30 months of noninvasive fetal RHD screening is presented. Cell-free DNA was extracted from 7192 plasma samples using a commercial kit, followed by an in-house qPCR to detect RHD exons 5 and 7, in addition to an amplification control. Valid results were obtained from 7072 samples, with 4515 (64%) fetuses typed RHD positive and 2556 (36%) fetuses being RHD negative. A total of 120 samples led to inconclusive results due to the presence of maternal or fetal RHD variants (46%), followed by women being serologically RH1 positive (37%), and technical issues (17%). One sample was typed false positive, possibly due to contamination. No false negative results were observed. We show that unnecessary administration of RHIG can be avoided for more than one third of RH1 negative pregnant women in Switzerland. This reduces the risks of exposure to a blood-derived product and conserves this limited resource to women in actual need.
PID Serval
serval:BIB_E59032A7A5C6
PMID
Open Access
Oui
Date de création
2023-11-06T12:34:09.537Z
Date de création dans IRIS
2025-05-21T05:54:49Z
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Nom
37893020_BIB_E59032A7A5C6.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by/4.0
Taille
577.66 KB
Format
Adobe PDF
PID Serval
serval:BIB_E59032A7A5C6.P001
URN
urn:nbn:ch:serval-BIB_E59032A7A5C68
Somme de contrôle
(MD5):523a8f7ca2a238605298d9ede51f8654