Titre
Evaluation of a rapid latex agglutination test (Directigen) for the direct detection of group A streptococci from throat swabs
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Kamm, W.
Auteure/Auteur
Bille, J.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0300-8126
Statut éditorial
Publié
Date de publication
1985-08
Volume
13
Numéro
4
Première page
179
Dernière page/numéro d’article
81
Peer-reviewed
Oui
Notes
Comparative Study
Journal Article --- Old month value: Jul-Aug
Journal Article --- Old month value: Jul-Aug
Résumé
A rapid group A beta-hemolytic streptococci (GAS) antigen detection test using latex agglutination (Directigen, Becton Dickinson) was compared with a conventional culture method for the direct detection of GAS from throat swabs. One throat specimen was collected from each of 229 patients. After standard inoculation onto sheep blood agar plates, all swabs were tested for GAS antigen. Of the 229 specimens tested, 46 (20%) were GAS-positive by culture. Direct latex agglutination agreed with culture in 222 of them (97%). The sensitivity of the test was 93% (43/46), the specificity 98% (179/183), the positive predictive value 91%, and the negative predictive value 98%. The detection of GAS antigen by coagglutination (Phadebact, Pharmacia) was also carried out. 81% (35/43) of the cultures and Directigen-positive specimens gave a positive coagglutination for GAS. 100% (179/179) of the cultures and Directigen-negative specimens were also negative by the coagglutination test. We conclude that the Directigen rapid latex agglutination test for the direct detection of GAS from throat swabs compares favorably with culture and has the advantage of providing same-day results.
Sujets
PID Serval
serval:BIB_9E7F57892993
PMID
Date de création
2008-02-11T11:39:37.007Z
Date de création dans IRIS
2025-05-20T22:10:20Z