Titre
Transcription of the human loricrin gene in vitro is induced by calcium and cell density and suppressed by retinoic acid
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Hohl, D.
Auteure/Auteur
Lichti, U.
Auteure/Auteur
Breitkreutz, D.
Auteure/Auteur
Steinert, P. M.
Auteure/Auteur
Roop, D. R.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0022-202X
Statut éditorial
Publié
Date de publication
1991-04
Volume
96
Numéro
4
Première page
414
Dernière page/numéro d’article
8
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr
Résumé
We have previously shown that loricrin is a major component of the cornified cell envelope (CE) expressed late in epidermal differentiation in the granular layers of normal skin. Normal human keratinocytes were cultured under various conditions and loricrin mRNA levels were assessed at various time points. Only Ca++ concentrations above 0.1 mM Ca++ were permissive for the expression of lori-crin mRNA. Maximal mRNA levels were found at 0.35 mM Ca++ and a critical cell density appeared to be required for optimal accumulation of loricrin transcripts. Retinoic acid (RA) at 10(-7) to 10(-9) M completely blocked Ca+(+)-induced loricrin mRNA synthesis when applied simultaneously. Furthermore, addition of RA to cultures already exposed to higher Ca++ levels resulted in the complete loss of loricrin mRNA within 48-72 h. So far, no other components of the CE have been shown to be suppressed by RA. However, similar patterns of expression were reported for filaggrin, a matrix protein also expressed late in epidermal differentiation. Therefore, we compared the mRNA levels of loricrin and filaggrin and found them to change in parallel in response to the various culture conditions. These results suggest that Ca++, cell density, and RA are crucial regulators of loricrin expression in vitro and that the transcriptional control of loricrin and filaggrin expression in the epidermis are closely coordinated.
Sujets
PID Serval
serval:BIB_010859A06D26
PMID
Open Access
Oui
Date de création
2008-01-25T15:36:18.932Z
Date de création dans IRIS
2025-05-20T15:13:16Z