Titre
Successful DNA typing of ultrafiltered urines used to detect EPO doping.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Castella, V.
Auteure/Auteur
Morerod, M.L.
Auteure/Auteur
Robinson, N.
Auteure/Auteur
Saugy, M.
Auteure/Auteur
Mangin, P.
Auteure/Auteur
Liens vers les personnes
ISSN
1878-0326
Statut éditorial
Publié
Date de publication
2007
Volume
1
Numéro
3-4
Première page
281
Dernière page/numéro d’article
282
Peer-reviewed
Oui
Langue
anglais
Résumé
Endogenous and exogenous erythropoietin (EPO) present in urine can be distinguished according to their isoelectric profiles. This methodology requires urine samples to be concentrated about 200 to 1000 times with manipulations that should remove most of the cells occurring in the original sample. In this study, we tried to obtain DNA profiles from 10 ultrafiltered urines (retentates) in order to evaluate whether a formal genetic identification was technically feasible. No nuclear DNA profiles could be established from retentates, despite 34 PCR-cycles amplifications. Contrastingly, mitochondrial DNA (mtDNA) profiles were obtained for 9 out of the 10 retentates. Apart from some particularities, retentate mtDNA profiles were all distinct and matched mtDNA profiles of corresponding reference samples.
PID Serval
serval:BIB_540222566C48
PMID
Date de création
2008-02-22T14:54:39.509Z
Date de création dans IRIS
2025-05-20T19:51:38Z