Titre
arcD, the first gene of the arc operon for anaerobic arginine catabolism in Pseudomonas aeruginosa, encodes an arginine-ornithine exchanger.
Type
article
Institution
Externe
Périodique
Auteur(s)
Verhoogt, H.J.
Auteure/Auteur
Smit, H.
Auteure/Auteur
Abee, T.
Auteure/Auteur
Gamper, M.
Auteure/Auteur
Driessen, A.J.
Auteure/Auteur
Haas, D.
Auteure/Auteur
Konings, W.N.
Auteure/Auteur
Liens vers les personnes
ISSN
0021-9193
Statut éditorial
Publié
Date de publication
1992
Volume
174
Numéro
5
Première page
1568
Dernière page/numéro d’article
1573
Langue
anglais
Résumé
In the absence of oxygen and nitrate, Pseudomonas aeruginosa metabolizes arginine via the arginine deiminase pathway, which allows slow growth on rich media. The conversion of arginine to ornithine, CO2, and NH3 is coupled to the production of ATP from ADP. The enzymes of the arginine deiminase pathway are organized in the arcDABC operon. The arcD gene encodes a hydrophobic polytopic membrane protein. Translocation of arginine and ornithine in membrane vesicles derived from an Escherichia coli strain harboring a recombinant plasmid carrying the arcD gene was studied. Arginine and ornithine uptake was coupled to the proton motive force with a bias toward the transmembrane electrical potential. Accumulated ornithine was readily exchangeable for external arginine or lysine. The exchange was several orders of magnitude faster than proton motive force-driven transport. The ArcD protein was reconstituted in proteoliposomes after detergent solubilization of membrane vesicles. These proteoliposomes mediate a stoichiometric exchange between arginine and ornithine. It is concluded that the ArcD protein is a transport system that catalyzes an electroneutral exchange between arginine and ornithine to allow high-efficiency energy conversion in the arginine deiminase pathway.
Sujets
PID Serval
serval:BIB_1BC6DF144780
PMID
Date de création
2008-01-25T16:01:12.749Z
Date de création dans IRIS
2025-05-20T16:43:24Z