Titre
Localization of epithelial sodium channel and aquaporin-2 in rabbit kidney cortex
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Auteur(s)
Loffing, J.
Auteure/Auteur
Loffing-Cueni, D.
Auteure/Auteur
Macher, A.
Auteure/Auteur
Hebert, S. C.
Auteure/Auteur
Olson, B.
Auteure/Auteur
Knepper, M. A.
Auteure/Auteur
Rossier, B. C.
Auteure/Auteur
Kaissling, B.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0363-6127
Statut éditorial
Publié
Date de publication
2000-04
Volume
278
Numéro
4
Première page
F530
Dernière page/numéro d’article
9
Peer-reviewed
Oui
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Research Support, Non-U.S. Gov't --- Old month value: Apr
Résumé
The amiloride-sensitive epithelial sodium channel (ENaC) and the vasopressin-dependent water channel aquaporin-2 (AQP2) mediate mineralocorticoid-regulated sodium- and vasopressin-regulated water reabsorption, respectively. Distributions of ENaC and AQP2 have been shown by immunohistochemistry in rats. Functional data from rabbits suggest a different distribution pattern of these channels than in rats. We studied, by immunohistochemistry in the rabbit kidney cortex, the distributions of ENaC and AQP2, in conjunction with marker proteins for distal segments. In rabbit cortex ENaC is restricted to the connecting tubule (CNT) cells and cortical collecting duct (CCD) cells. The intracellular distribution of ENaC shifts from the apical membrane in the most upstream CNT cells to a cytoplasmic location further downstream in the CNT and in the CCD cells. AQP2 is detected in the CCD cells exclusively. The anatomic subdivisions in the rabbit distal nephron coincide exactly with distributions of apical transport systems. The differences between rabbits and rats in the distribution patterns of ENaC and AQP2 may explain functional differences in renal salt and water handling between these species.
Sujets
PID Serval
serval:BIB_AC4AB35ED1EC
PMID
Date de création
2008-01-24T12:00:45.783Z
Date de création dans IRIS
2025-05-21T02:22:42Z