Titre
Influence of fixatives and embedding media on immunolabelling of freeze-substituted cells.
Type
article
Institution
Externe
Périodique
Scanning Microscopy. Supplement
Auteur(s)
Schwarz, H.
Auteure/Auteur
Humbel, B.M.
Auteure/Auteur
Liens vers les personnes
ISSN
0892-953X
Statut éditorial
Publié
Date de publication
1989
Volume
3
Première page
57
Dernière page/numéro d’article
63; discussion 63-4
Langue
anglais
Résumé
Fixatives and embedding media are known to alter antigen epitopes and reduce antibody recognition unpredictably. Every antigen-antibody interaction may be influenced differently. In this study we concentrate on the influence of fixatives, commonly used in immuno-electron microscopy, the dehydration process, and the embedding media Lowicryl HM20 and K4M. The results obtained with our model system show that: the lesser chemical fixation, the more intense the labelling, and that dehydration by freeze-substitution preserves the antigenicity better than dehydration by progressive lowering of temperature, PLT, and finally that there is no obvious difference in labelling between the apolar Lowicryl HM20 and the polar Lowicryl K4M.
PID Serval
serval:BIB_89E06F03D6B8
PMID
Date de création
2012-10-18T12:07:34.017Z
Date de création dans IRIS
2025-05-21T00:39:19Z