Titre
Human clusterin gene expression is confined to surviving cells during in vitro programmed cell death
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
French, L. E.
Auteure/Auteur
Wohlwend, A.
Auteure/Auteur
Sappino, A. P.
Auteure/Auteur
Tschopp, J.
Auteure/Auteur
Schifferli, J. A.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0021-9738
Statut éditorial
Publié
Date de publication
1994-02
Volume
93
Numéro
2
Première page
877
Dernière page/numéro d’article
84
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Résumé
Clusterin is a serum glycoprotein endowed with cell aggregating, complement inhibitory, and lipid binding properties, and is also considered as a specific marker of dying cells, its expression being increased in various tissues undergoing programmed cell death (PCD). However, no study has so far directly shown that cells expressing clusterin in these tissues are actually apoptotic as defined by morphological and biochemical criteria. We have studied cellular clusterin gene expression in vitro using three different models of PCD: (a) ultraviolet B (UV-B) irradiation of human U937, HeLa, and A431 cell lines, (b) in vitro aging of human peripheral blood neutrophils (PMNs), and (c) dexamethasone-induced cell death of the human lymphoblastoid cell line CEM-C7. In all three models, the classical morphological and biochemical features of PCD observed did not correlate with an increase, but with either a marked decrease or an absence of clusterin gene expression as assessed by Northern blot analysis. In situ hybridization of U937 and A431 cells after UV-B irradiation revealed, in addition, that only morphologically normal cells that are surviving continue to express the clusterin gene. Our results demonstrate that in the human myeloid, lymphoid, and epithelial cell types studied, clusterin gene expression is not a prerequisite to their death by apoptosis. In addition, and most interestingly, in situ hybridization of U937 and A431 cells revealed that only surviving cells express the clusterin gene after the induction of PCD, thus providing novel evidence suggesting that clusterin may be associated with cell survival within tissues regressing as a consequence of PCD.
Sujets
PID Serval
serval:BIB_EDD4BE6837D3
PMID
Open Access
Oui
Date de création
2008-01-24T14:18:14.919Z
Date de création dans IRIS
2025-05-21T06:23:41Z