Titre
Pravastatin limits endothelial activation after irradiation and decreases the resulting inflammatory and thrombotic responses.
Type
article
Institution
Externe
Périodique
Auteur(s)
Gaugler, M.H.
Auteure/Auteur
Vereycken-Holler, V.
Auteure/Auteur
Squiban, C.
Auteure/Auteur
Vandamme, M.
Auteure/Auteur
Vozenin-Brotons, M.C.
Auteure/Auteur
Benderitter, M.
Auteure/Auteur
Liens vers les personnes
ISSN
0033-7587
Statut éditorial
Publié
Date de publication
2005-05
Volume
163
Numéro
5
Première page
479
Dernière page/numéro d’article
487
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
Endothelial dysfunction has been implicated in the pathogenesis of atherosclerosis, fibrosis and vascular occlusion after radiation therapy. Statins have been reported to improve endothelial function; however, this beneficial effect on endothelial cells has never been investigated after irradiation. Therefore, using human microvascular endothelial cells from lung that had been irradiated with 5 or 10 Gy, we assessed the effect of pravastatin on endothelial activation by ELISA, cell-ELISA and electrophoretic mobility shift assay and increased blood-endothelial cell interactions by a flow adhesion assay. Pravastatin inhibited the overproduction of monocyte chemoattractant protein 1, IL6 and IL8 and the enhanced expression of intercellular adhesion molecule 1 but had no effect on platelet-endothelial cell adhesion molecule 1 expression. Moreover, pravastatin down-regulated the radiation-induced activation of the transcription factor activator protein 1 but not of nuclear factor-kappaB. Finally, an inhibition by pravastatin of increased adhesion of leukocytes and platelets to irradiated endothelial cells was observed. The effect of pravastatin was maintained up to 14 days after irradiation and was reversed by mevalonate. Pravastatin exerts persistent anti-inflammatory and anti-thrombotic effects on irradiated endothelial cells. Statins may be considered in therapeutic strategies for the management of patients treated with radiation therapy.
Sujets
PID Serval
serval:BIB_C4418B0D6853
PMID
Date de création
2018-04-27T14:48:05.915Z
Date de création dans IRIS
2025-05-21T03:31:51Z