Titre
Insights into Mobile Genetic Elements of the Biocide-Degrading Bacterium Pseudomonas nitroreducens HBP-1.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Carraro, N.
Auteure/Auteur
Sentchilo, V.
Auteure/Auteur
Polák, L.
Auteure/Auteur
Bertelli, C.
Auteure/Auteur
van der Meer, J.R.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
2073-4425
Statut éditorial
Publié
Date de publication
2020-08-12
Volume
11
Numéro
8
Première page
930
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
The sewage sludge isolate Pseudomonas nitroreducens HBP-1 was the first bacterium known to completely degrade the fungicide 2-hydroxybiphenyl. PacBio and Illumina whole-genome sequencing revealed three circular DNA replicons: a chromosome and two plasmids. Plasmids were shown to code for putative adaptive functions such as heavy metal resistance, but with unclarified ability for self-transfer. About one-tenth of strain HBP-1's chromosomal genes are likely of recent horizontal influx, being part of genomic islands, prophages and integrative and conjugative elements (ICEs). P. nitroreducens carries two large ICEs with different functional specialization, but with homologous core structures to the well-known ICEclc of Pseudomonas knackmussii B13. The variable regions of ICEPni1 (96 kb) code for, among others, heavy metal resistances and formaldehyde detoxification, whereas those of ICEPni2 (171 kb) encodes complete meta-cleavage pathways for catabolism of 2-hydroxybiphenyl and salicylate, a protocatechuate pathway and peripheral enzymes for 4-hydroxybenzoate, ferulate, vanillin and vanillate transformation. Both ICEs transferred at frequencies of 10 <sup>-6</sup> -10 <sup>-8</sup> per P. nitroreducens HBP-1 donor into Pseudomonas putida, where they integrated site specifically into tRNA <sup>Gly</sup> -gene targets, as expected. Our study highlights the underlying determinants and mechanisms driving dissemination of adaptive properties allowing bacterial strains to cope with polluted environments.
PID Serval
serval:BIB_206C29759190
PMID
Open Access
Oui
Date de création
2020-08-28T06:47:42.489Z
Date de création dans IRIS
2025-05-20T13:50:07Z
Fichier(s)![Vignette d'image]()
En cours de chargement...
Nom
32806781_BIB_206C29759190.pdf
Version du manuscrit
published
Licence
https://creativecommons.org/licenses/by/4.0
Taille
12.31 MB
Format
Adobe PDF
PID Serval
serval:BIB_206C29759190.P001
URN
urn:nbn:ch:serval-BIB_206C297591900
Somme de contrôle
(MD5):059ad0f8f1ca42924c79f1d35bd7f245