Titre

Vesicular Glutamate Transporters in Astrocytes as Potential New Therapeutic Targets: Astrocyte-targeted Viral Vectors Expressing Inhibitory Nanobodies.

Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
De Ceglia, R.
Auteure/Auteur
Volterra, A.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0009-4293
Statut éditorial
Publié
Date de publication
2022-12-21
Volume
76
Numéro
12
Première page
1033
Dernière page/numéro d’article
1038
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Astrocytes, the main Central Nervous System (CNS) glial cell type, actively release transmitters, including glutamate, and thereby participate in physiological brain information processing. However, dysregulated transmitter release from astrocytes can contribute to CNS disease pathogenesis and progression. Therefore, targeting astrocyte glutamate release is a promising new therapeutic strategy in hyper-glutamatergic brain conditions, as it does not directly block glutamatergic neurotransmission. Basing on the evidence that astrocytes express Vesicular Glutamate Transporters (VGLUT), in collaboration with other NCCR TransCure partners, we developed an innovative approach for astrocyte-selective delivery of nanobodies inhibiting VGLUT. We inserted the anti-VGLUT nanobody constructs in astrocyte-directed viral vectors that were administered peripherally, crossed the blood-brain-barrier and led to successful cell-specific CNS-wide expression of the nanobodies.
Sujets

Astrocyte

Cell-specific viral v...

Nanobody

Vglut

VGLUT

PID Serval
serval:BIB_67DEC349B3D1
DOI
PMID
Permalien
Open Access
Oui
Date de création
2023-12-15T13:43:40.958Z
Date de création dans IRIS
2025-05-20T21:52:32Z
Fichier(s)
En cours de chargement...
Vignette d'image
Nom

38069799.pdf

Version du manuscrit

published

Licence

https://creativecommons.org/licenses/by/4.0

Taille

422 KB

Format

Adobe PDF

PID Serval

serval:BIB_67DEC349B3D1.P001

URN

urn:nbn:ch:serval-BIB_67DEC349B3D18

Somme de contrôle

(MD5):45599e6040d855d0db0f264c4cb13c32