Titre
Proteome analysis of human plasma and amniotic fluid by Off-Gel isoelectric focusing followed by nano-LC-MS/MS
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Michel, P. E.
Auteure/Auteur
Crettaz, D.
Auteure/Auteur
Morier, P.
Auteure/Auteur
Heller, M.
Auteure/Auteur
Gallot, D.
Auteure/Auteur
Tissot, J. D.
Auteure/Auteur
Reymond, F.
Auteure/Auteur
Rossier, J. S.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0173-0835
Statut éditorial
Publié
Date de publication
2006-03
Volume
27
Numéro
5-6
Première page
1169
Dernière page/numéro d’article
81
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Résumé
This paper presents a comparative proteomic analysis of human maternal plasma and amniotic fluid (AF) samples from the same patient at term of pregnancy in order to find specific AF proteins as markers of premature rupture of membranes, a complication frequently observed during pregnancy. Maternal plasma and the corresponding AF were immunodepleted in order to remove the six most abundant proteins before the systematic analysis of their protein composition. The protein samples were then fractionated by IEF Off-Gel electrophoresis (OGE), digested and analyzed with nano-LC-MS/MS separation, revealing a total of 73 and 69 proteins identified in maternal plasma and AF samples, respectively. The proteins identified in AF have been compared to those identified in the mother plasma as well as to the reference human plasma protein list reported by Anderson et al. (Mol. Cell. Proteomics 2004, 3, 311-326). This comparison showed that 26 proteins were exclusively present in AF and not in plasma among which 10 have already been described to be placenta or pregnancy specific. As a further validation of the method, plasma proteins fractionated by OGE and analysed by nano-LC-MS/MS have been compared to the Swiss 2-D PAGE reference map by reconstructing a map that matches 2-D gel and OGE experimental data. This representation shows that 36 of 49 reference proteins could be identified in both data sets, and that isoform shifts in pI are well conserved in the OGE data sets.
Sujets
PID Serval
serval:BIB_926B241627CC
PMID
Date de création
2008-01-25T14:34:45.215Z
Date de création dans IRIS
2025-05-21T01:16:28Z