Titre
Calf rennet lysozyme
Type
abstract de conférence/colloque
Institution
Externe
Série
Experientia
Auteur(s)
Pahud, J.J.
Auteure/Auteur
Schwarz, K.
Auteure/Auteur
Meyer, I.
Auteure/Auteur
Widmer, F.
Auteure/Auteur
Liens vers les personnes
Titre du livre ou conférence/colloque
12th annual meeting of the Union of Swiss societies of experimental biology
Adresse
Basel, Switzerland, March 13-14, 1980
ISBN
0014-4754
Statut éditorial
Publié
Date de publication
1980
Volume
36
Première page
729
Peer-reviewed
Oui
Langue
anglais
Résumé
A strong lytic activity against Micrococcus lysodeikticus cell walls was detected in calf rennet. Purification was carried out using ion exchange, electrofocusing and gel-filtration. Hydrolysis of the bacterial peptidoglycan by the isolated enzyme released only reducing groups, thus indicating true glycolytic activity. Comparative tests with hen egg white lysozyme suggested a similar mode of action. - Multiple enzyme forms were revealed, with mol.wts of ca. 15,000, pH optima of ca. 5.0, and pl's ranging from 6.5 to 7.8. The enzyme exhibited remarkable stability against heat and pH conditions ranging from 2 to 9. Significant chitinase activity was observed at pH 5.0. - Specifie antisera revealed sharp precipitation arcs in the ß- to y-electrophoretic region, when diffused against the most acidic or basic molecular forms, respectively. - The rennet enzyme differs markedly from the bovine milk lysozyme described only by Chaudan et al. (BBA 110, 389, 1965).
PID Serval
serval:BIB_477DAE170CF0
Date de création
2015-08-13T06:53:31.051Z
Date de création dans IRIS
2025-05-20T19:26:20Z
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BIB_477DAE170CF0.P001.pdf
Version du manuscrit
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PID Serval
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