Titre
Characterization of the non-functional Fas ligand of gld mice
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Hahne, M.
Auteure/Auteur
Peitsch, M. C.
Auteure/Auteur
Irmler, M.
Auteure/Auteur
Schroter, M.
Auteure/Auteur
Lowin, B.
Auteure/Auteur
Rousseau, M.
Auteure/Auteur
Bron, C.
Auteure/Auteur
Renno, T.
Auteure/Auteur
French, L.
Auteure/Auteur
Tschopp, J.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
0953-8178
Statut éditorial
Publié
Date de publication
1995-09
Volume
7
Numéro
9
Première page
1381
Dernière page/numéro d’article
6
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Research Support, Non-U.S. Gov't --- Old month value: Sep
Résumé
Mice homozygous for either the gld or lpr mutation develop autoimmune diseases and progressive lymphadenopathy. The lpr mutation is characterized by the absence of functional Fas, whereas gld mice exhibit an inactive FasL due to a point mutation proximal to the extracellular C-terminus. The structural repercussions of this amino acid substitution remain unknown. Here we report that FasL is expressed at similar levels on the surface of activated T lymphocytes from gld and wild-type mice. Using a polyclonal anti-FasL antibody, indistinguishable amounts of a 40 kDa protein are detected in both gld and wild-type splenocytes. The molecular model of FasL, based on the known structure of TNF-alpha, predicts that the Phe --> Leu gld mutation is located at the protomer interface which is close to the FasR interaction site. We conclude that the gld mutation allows normal FasL biosynthesis, surface expression and oligomerization, but induces structural alterations to the Fas binding region leading to the phenotypic changes observed.
Sujets
PID Serval
serval:BIB_0BA9B77E7F43
PMID
Open Access
Oui
Date de création
2008-01-24T14:19:41.503Z
Date de création dans IRIS
2025-05-20T16:48:09Z
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Nom
REF.pdf
Version du manuscrit
published
Taille
6.88 MB
Format
Adobe PDF
PID Serval
serval:BIB_0BA9B77E7F43.P001
URN
urn:nbn:ch:serval-BIB_0BA9B77E7F431
Somme de contrôle
(MD5):5505a27c1f81f059365925b0f3521627