Titre
Hypoxia and hypocapnia storage of γ-irradiated red cell concentrates.
Type
article
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Blood transfusion = Trasfusione del sangue
Auteur(s)
Bardyn, M.
Auteure/Auteur
Crettaz, D.
Auteure/Auteur
Borlet, M.
Auteure/Auteur
Längst, E.
Auteure/Auteur
Martin, A.
Auteure/Auteur
Abonnenc, M.
Auteure/Auteur
Tissot, J.D.
Auteure/Auteur
Dunham, A.
Auteure/Auteur
Yoshida, T.
Auteure/Auteur
Prudent, M.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
2385-2070
Statut éditorial
Publié
Date de publication
2021-07
Volume
19
Numéro
4
Première page
300
Dernière page/numéro d’article
308
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
Publication Status: ppublish
Publication Status: ppublish
Résumé
γ-irradiation is used to treat red blood cell (RBC) concentrates (RCCs) transfused to immunosuppressed patients. This treatment damages RBCs and increases storage lesions. Several studies have shown the beneficial effect of reducing O <sub>2</sub> content during RBC storage. The present research work investigated the effect of γ-irradiation on RCCs stored under normal and hypoxia/hypocapnia conditions.
O <sub>2</sub> concentration (measured as oxyhaemoglobin fraction, sO <sub>2</sub> ) and ABO-matched RCCs from whole blood donations, leukoreduced and prepared in phosphate, adenine, glucose, guanosine, saline and mannitol (PAGGSM) were pooled and split in two identical RCCs within 24 h post donation. One bag (Hx) was submitted to O <sub>2</sub> and CO <sub>2</sub> adsorption for 3 h on an orbital shaker at 22±2 °C and then transferred to a storage bag impermeable to gas. The other bag (Ctrl) was left as it was. The two bags were then stored at 4 °C. γ-irradiation (25 Gy) was applied at day 2 or 14, and the RCCs were stored until day 43. Different parameters (metabolites, haemolysis, morphology) were measured.
Starting sO <sub>2</sub> values were 63.7±18.4% (n=12) in Ctrl and 20.8±9.8% (n=12) in Hx bags, and reached 90.8±9.1% and 6.6±5.9% at day 43, respectively. As expected, an increase in glycolysis rate was observed after deoxygenation. Extracellular potassium concentrations were identical and reached around 70 mM at expiry with an irradiation-dependent kinetic release. No difference in haemolysis was observed after irradiation on day 2 in either group (<0.40%, p>0.9999). When irradiated at day 14, haemolysis was lower (p=0.033) in RCCs under hypoxia at the end of storage (day 28, 0.67±0.16%) compared to control (1.06±0.33%). Percentages of spherocytes were lower under hypoxia.
The storage under hypoxia provided equivalent storage when RCCs were irradiated at day 2 and was advantageous when irradiated at day 14. In summary, O <sub>2</sub> -depletion of RCCs enable a better storage of RBCs, particularly when late irradiation is applied.
O <sub>2</sub> concentration (measured as oxyhaemoglobin fraction, sO <sub>2</sub> ) and ABO-matched RCCs from whole blood donations, leukoreduced and prepared in phosphate, adenine, glucose, guanosine, saline and mannitol (PAGGSM) were pooled and split in two identical RCCs within 24 h post donation. One bag (Hx) was submitted to O <sub>2</sub> and CO <sub>2</sub> adsorption for 3 h on an orbital shaker at 22±2 °C and then transferred to a storage bag impermeable to gas. The other bag (Ctrl) was left as it was. The two bags were then stored at 4 °C. γ-irradiation (25 Gy) was applied at day 2 or 14, and the RCCs were stored until day 43. Different parameters (metabolites, haemolysis, morphology) were measured.
Starting sO <sub>2</sub> values were 63.7±18.4% (n=12) in Ctrl and 20.8±9.8% (n=12) in Hx bags, and reached 90.8±9.1% and 6.6±5.9% at day 43, respectively. As expected, an increase in glycolysis rate was observed after deoxygenation. Extracellular potassium concentrations were identical and reached around 70 mM at expiry with an irradiation-dependent kinetic release. No difference in haemolysis was observed after irradiation on day 2 in either group (<0.40%, p>0.9999). When irradiated at day 14, haemolysis was lower (p=0.033) in RCCs under hypoxia at the end of storage (day 28, 0.67±0.16%) compared to control (1.06±0.33%). Percentages of spherocytes were lower under hypoxia.
The storage under hypoxia provided equivalent storage when RCCs were irradiated at day 2 and was advantageous when irradiated at day 14. In summary, O <sub>2</sub> -depletion of RCCs enable a better storage of RBCs, particularly when late irradiation is applied.
PID Serval
serval:BIB_4338B49A63C1
PMID
Open Access
Oui
Date de création
2020-09-22T10:01:22.897Z
Date de création dans IRIS
2025-05-20T14:38:35Z