Titre
Listen to Your Gut: Key Concepts for Bioengineering Advanced Models of the Intestine.
Type
synthèse (review)
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Périodique
Auteur(s)
Cameron, O.
Auteure/Auteur
Neves, J.F.
Auteure/Auteur
Gentleman, E.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
ISSN
2198-3844
Statut éditorial
Publié
Date de publication
2024-02
Volume
11
Numéro
5
Première page
e2302165
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Review
Publication Status: ppublish
Publication Status: ppublish
Résumé
The intestine performs functions central to human health by breaking down food and absorbing nutrients while maintaining a selective barrier against the intestinal microbiome. Key to this barrier function are the combined efforts of lumen-lining specialized intestinal epithelial cells, and the supportive underlying immune cell-rich stromal tissue. The discovery that the intestinal epithelium can be reproduced in vitro as intestinal organoids introduced a new way to understand intestinal development, homeostasis, and disease. However, organoids reflect the intestinal epithelium in isolation whereas the underlying tissue also contains myriad cell types and impressive chemical and structural complexity. This review dissects the cellular and matrix components of the intestine and discusses strategies to replicate them in vitro using principles drawing from bottom-up biological self-organization and top-down bioengineering. It also covers the cellular, biochemical and biophysical features of the intestinal microenvironment and how these can be replicated in vitro by combining strategies from organoid biology with materials science. Particularly accessible chemistries that mimic the native extracellular matrix are discussed, and bioengineering approaches that aim to overcome limitations in modelling the intestine are critically evaluated. Finally, the review considers how further advances may extend the applications of intestinal models and their suitability for clinical therapies.
PID Serval
serval:BIB_927434AB8368
PMID
Open Access
Oui
Date de création
2023-12-01T09:55:47.693Z
Date de création dans IRIS
2025-05-21T03:43:33Z
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Nom
Cameron et al Author Accepted version.pdf
Version du manuscrit
postprint
Licence
https://creativecommons.org/licenses/by/4.0
Taille
7.34 MB
Format
Adobe PDF
PID Serval
serval:BIB_927434AB8368.P001
URN
urn:nbn:ch:serval-BIB_927434AB83681
Somme de contrôle
(MD5):43702cf5a7e16fbd8530a62ae5f1555b