Titre
CO-EXPRESSION OF GCDH AND OAT1 IN NEURONS AND PROXIMAL TUBULE CELLS
Type
poster de conférence/colloque
Institution
UNIL/CHUV/Unisanté + institutions partenaires
Série
Journal of Inherited Metabolic Diseases
Auteur(s)
Ballhausen, D.
Auteure/Auteur
Jafari, P.
Auteure/Auteur
Bonafe, L.
Auteure/Auteur
Braissant, O.
Auteure/Auteur
Liens vers les personnes
Liens vers les unités
Titre du livre ou conférence/colloque
Annual Symposium of the Society for the Study of Inborn Errors of Metabolism
Adresse
Geneva, Switzerland, August 30-September 2, 2011
ISBN
0141-8955
Statut éditorial
Publié
Date de publication
2011
Volume
34
Première page
S141
Langue
anglais
Notes
Document Type:Meeting Abstract
Résumé
Tissue-specific expression studies of Glutaryl-CoA dehydrogenase (Gcdh)
in adult rats revealed expression in the whole rat brain, almost exclusively
in neurons, and surprisingly high expression in the juxtamedullar cortex of
the kidney. The organic anion transporter 1 (OAT1) mediates basolateral
uptake of glutarate derivatives from proximal tubule cells and contributes
to their renal clearance. In brain, OAT1 is expressed at the choroid plexus,
in neurons of cortex and hippocampus. We hypothesized that Gcdh and
Oat1 are co-expressed in the same cells in kidney and brain and analyzed
their mRNA expression by in situ hybridization on cryosections of adult rat
brain, kidney and liver. In brain, Gcdh and Oat1 were found co-expressed
in most neurons. Only the Purkinje neurons of the cerebellum were found
to be Oat1 negative. In the kidney Gcdh and Oat1 are widely co-expressed
with a specific high expression in proximal tubule cells. In conclusion
there seems to be a functional coupling of Gcdh and Oat1 on a renal and
neuronal level. Further studies are ongoing to confirm these findings in
human tissues.
in adult rats revealed expression in the whole rat brain, almost exclusively
in neurons, and surprisingly high expression in the juxtamedullar cortex of
the kidney. The organic anion transporter 1 (OAT1) mediates basolateral
uptake of glutarate derivatives from proximal tubule cells and contributes
to their renal clearance. In brain, OAT1 is expressed at the choroid plexus,
in neurons of cortex and hippocampus. We hypothesized that Gcdh and
Oat1 are co-expressed in the same cells in kidney and brain and analyzed
their mRNA expression by in situ hybridization on cryosections of adult rat
brain, kidney and liver. In brain, Gcdh and Oat1 were found co-expressed
in most neurons. Only the Purkinje neurons of the cerebellum were found
to be Oat1 negative. In the kidney Gcdh and Oat1 are widely co-expressed
with a specific high expression in proximal tubule cells. In conclusion
there seems to be a functional coupling of Gcdh and Oat1 on a renal and
neuronal level. Further studies are ongoing to confirm these findings in
human tissues.
PID Serval
serval:BIB_689ED77047BD
Date de création
2014-02-14T16:27:27.891Z
Date de création dans IRIS
2025-05-20T21:24:43Z