Titre
Yersinia enterocolitica type III secretion injectisomes form regularly spaced clusters, which incorporate new machines upon activation.
Type
article
Institution
Externe
Périodique
Auteur(s)
Kudryashev, M.
Auteure/Auteur
Diepold, A.
Auteure/Auteur
Amstutz, M.
Auteure/Auteur
Armitage, J.P.
Auteure/Auteur
Stahlberg, H.
Auteure/Auteur
Cornelis, G.R.
Auteure/Auteur
Liens vers les personnes
ISSN
1365-2958
Statut éditorial
Publié
Date de publication
2015-03
Volume
95
Numéro
5
Première page
875
Dernière page/numéro d’article
884
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
Bacterial type III secretion systems or injectisomes are multiprotein complexes directly transporting bacterial effector proteins into eukaryotic host cells. To investigate the distribution of injectisomes in the bacterium and the influence of activation of the system on that distribution, we combined in vivo fluorescent imaging and high-resolution in situ visualization of Yersinia enterocolitica injectisomes by cryo-electron tomography. Fluorescence microscopy showed the injectisomes as regularly distributed spots around the bacterial cell. Under secreting conditions (absence of Ca(2+) ), the intensity of single spots significantly increased compared with non-secreting conditions (presence of Ca(2+) ), in line with an overall up-regulation of expression levels of all components. Single injectisomes observed by cryo-electron tomography tended to cluster at distances less than 100 nm, suggesting that the observed fluorescent spots correspond to evenly distributed clusters of injectisomes, rather than single injectisomes. The up-regulation of injectisome components led to an increase in the number of injectisomes per cluster rather than the formation of new clusters. We suggest that injectisome clustering may allow more effective secretion into the host cells.
PID Serval
serval:BIB_568D32881F43
PMID
Open Access
Oui
Date de création
2023-06-09T14:02:58.910Z
Date de création dans IRIS
2025-05-20T16:31:47Z